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Genetic Variation Of Endosperm Storage Protein And Agronomic Traits In Triticum Carthlicum Nevski

Posted on:2007-03-01Degree:MasterType:Thesis
Country:ChinaCandidate:P P ZhuangFull Text:PDF
GTID:2133360185480319Subject:Crop Genetics and Breeding
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Triticum carthlicum Nevski, an important tetroploid species in Triticum, is the important genetic resources for the improvement of bread wheat. With valuable gene resources, it has some desirable characters such as high tillering ability, good fecundity, high protein content and good resistance to powdery mildew, dustbrand and stem rust, also with tolerance to low temprature and preharvest sprouting. To exploit the new genetic resources for utilizing excellent traits efficiently, the genetic variations of storage proteins and the main agronomic traits were analyzed and evaluated. The main results were described as follows:1. Genetic variations of gliadins among 85 persian wheat accessions from 15 countries were evaluated by A-PAGE. Higher genetic variability was observed. A total of 53 polymorphism bands were detected, and 66 gliadin patterns were found, among which 28 accessions shared 9 gliadin patterns and 57 accessions had unique gliadin patterns. The mean genetic similarity (GS) was 0.542, ranging from 0.175 to 1.000. All accessions were clustered into 6 groups. The clustering analysis results suggested that the genetic relationship among T. carthlicum accessions based on the gliadins were associated with its geographical distribution.2. The results based on SDS-PAGE showed that a total of 8 alleles and 6 subunitcombinations were found at Glu-1 of T. carthlicum. At Glu-Al, the frequency of...
Keywords/Search Tags:Triticum carthlicum Nevski, Gliadin, Glutenin, Agronomic traits, A-PAGE, SDS-PAGE
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