Identification Of NDV Strain Isolated In Shandong Province And Molecular Cloning And Analysis Of The Fusion And Haemaglutinin-Neuraminidase Gene

Newcastle disease (ND) is regarded worldwide as one of the most devastating diseases of poultry. Its causative agent, Newcastle disease virus (NDV), is classified as a member of the order Mononegavirales family, Paramyxovirinae subfamily, Paramyxovirinae genus rubulavirus. Because of the severe nature of the disease and the associated consequences, ND is included as an Office International des Epizooties (OIE) list A disease. In the past two decades, an intensive vaccination program against ND has been practiced in both large-scale poultry operations and village poultry farming. Disease outbreaks occur infrequently in some vaccinated flocks, however, infections of atypical Newcastle disease in the chicken flocks have been more and more frequently reported since the late 1990s.Even some chicken flocks with high antibody level can also suffer ND. Some scholar think that the emergence of new genotypes or subgenotypes could be responsible for ND outbreak in vaccinated flocks. Especially when the emergence of variant NDV of goose origin was found, people are more and more interest in mechanism of the atypical Newcastle disease caused by virulent strain.Based on the prevalence condition of NDV, One Newcastle disease virus (named ShD-5-04) isolated from Shandong current outbreak ND chicken farms that only showed egg dropping and without any typical ND symptom...