Genetic Diversity Of Different Populations Of Patinopecten Yessoensis Using AFLP And SSR Molecular Markers

Abstract: Patinopecten yessoensis belongs to phylum Mollusa, class Lamellibranchia, subclass Pterimorphia Pectinidae, genus Mizuhopecten, which was native to Japan and Russa. After introduced in 1981, it mainly distributed in coast of Shandong and Liaoning Province from China, and has high output. In recent years, for the reason of human breeding, there has come out a series of problems of the Patinopecten yessoensis populations, such as the increasing of hermaphrodite, lower survival rate, declining of shelf inbreeding, and so on. To increase the output and the quality of the breeding Patinopecten yessoensis, ensure the sustainable development of the shell's breeding Project, it has been a growing interest issues of improving the quality, increasing the genetic diversity of the Patinopecten yessoensis. DNA molecular marker is able to express genetic information accurately for its excellence of stability, accuracy and security. In this paper, Three wild populations (QSX, HSW, LSK) and four breeding populations (ZZD, XCS, GLD, LS) of Patinopecten yessoensis were studied by means of AFLP and SSR (microsatellite ) molecular marker techniques. We hope that these results will provide the theory basis for inherited breeding of Patinopecten yessoensi.One hundred and sixty-eight Patinopecten yessoensi from seven populations (twenty-four individuals per population) were analyzed using seven AFLP primer combinations and three SSR primers respectively. AFLP and SSR banding patterns were transformed into binary data and matrices were processed by Popgene32 program. Using the data obtained, the genetic diversity and the genetic structure were estimated, the similarity coefficient was calculated and UPGMA clustering analyzed on computer among these Patinopecten yessoensi varieties. Results of two techniques are as follow:With the test of polymorphism for AFLP markers, total 450 loci were amplified using 7 primer combinations, and 424 were polymorphic loci. On average, 60.6 polymorphic bands are amplified by one primer combination. As for the SSR prmers, 21 alleles were amplified with 7 alleles each one. Both of the two techniques showed higher genetic diversity in all populations. Genetic parameters calculated by SSR technique were obviously higher than that of AFLP technique. The effectic alleles number (Ne) of SSR and AFLP were 1.4091 and 2.6412, observed heterozygosity ranged from 0.71 to 0.90 across populations for SSRs and from 0.2200 to 0.2561 for...