| The in vitro micropropagation techonique of chrysanthemum has advantages of artificial controlled enrironment factor,short reproduction period,fast speed,whole-year production,saving material.It started a new way for chrysanthemum seedling production in factory.The tissue culture process of cut chrysanthemum 'shang hai zao bai' was reseached in this paper,the cost reduction and reproduction rate raise was also discussed.It turned out reproduction rete of chrysanthemum was raised,the regular,non-plant diseases and insect pests could be offered to the market.Shoot tip were used as explants to study the effects of different sterilization methods, illumination intensity and concentrations of 6-BA and NAA on shoot propagation. Rooting with perlite, vermiculite and river sand instead of agar was studied. In the process of preliminary multiplication, the effect of using 0.1% HgCl2 in sterilization of explants was better than using saturated bleaching solution. The highest multiplication rate was found under whole illumination. MS media with 6-BA from 0.2 mg·L-1 to 5 mg·L-1 and NAA from 0.05 mg·L-1 to 2 mg·L-1 were tested and as a result, the highest differentiation rate and the multiplication rate was reached with 0.2 mg·L-1 6-BA, 0.05 mg·L-1 NAA.In the process of rooting, MS medium with NAA from 0.1 mg·L-1 to 1.0 mg·L-1 were tried on and as a result in MS media with NAA 0.1 mg·L-1 plantlet formed roots in only 4 days. The rate of rooting was 100%. When plantlet cultured in MS, 1/2MS, 1/4MS, 1/4MS with NAA 0:1 mg·L-1 was the most suitable media for rooting and the root was vigorous The rate of rooting was 100%. And the ratio of vermiculite: perlite=2:1 gave the best rooting with rooting ratio as high as 100%.The rooted plantlets were removed to plastic containers filled with soil, 100%survival could be reached.
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