Genetic Diversity And Its Potential Utilization Of Grain Trait And Storage Protein In Triticum Dicoccoides

Triticum dicoccoides is a second genetic resource of Triticum aestivum L. It had been considered as useful genetic resources for the improvement of wheat because of the excellent trais such as high protein content, genetic diversity of storage protein, and the resistance rust, powdery mildew and ustilago, etc. In this paper, the grain traits including protein content, 1000-grain weight, width and length of 110 accessions of wild emmer from Israel were studied. PF and PR were designed based on the highly conserved distal sequences of 5' and 3' ends of known LMW-GS genes, and utilized to amplify LMW-GS genes from Triticum dicoccoides accession D42 with the highest protein content and D81 with the lowest protein content. Consequently, their second structures of deduced amino acids sequences were predicted. The main results were as follows:1. Abundant genetic diversities in the protein content, 1000-grain weight, length, width, and length × width of wide emmer were found. The protein content ranged from 13.17% to 27.20%, with the mean of 19.89%. There were 68.2% of accessions with protein content over 18%. The 1000-grain weight varied from 7.95 to 42.7g, with the mean of 20.36g. The length of grain was 8.11~12.48mm, with the mean of 10.69mm and 80.91% of accessions over 10 mm. The width of grain varied from 1.46 to 2.89mm, the average was at 2.10 mm. The length x width was 11.92mm~2~31.28mm~2, with the mean of 22.57mm~2, and 71.82% of accessions over 20 mm~2.2. The correlation analysis showed that protein content was significantly negative...