I Study On The Changes Of Serum Lactate Dehydrogenase And Neuron-specific Enolase In Patients With Myeloproliferative Tumors. II Study On The Expression Of Neuron - Specific Enolase In Human Leukemia Cell Lines K562 And K562 / A02

OBJECTIVE To study the levels and clinical significance about serum glycolytic enzyme lactate dehydrogenase (LDH) and neuron-specific enolase (NSE) with polycythemia vera (PV), essential thrombocythemia (ET), primary myelofibrosis (PMF).METHODS Serum LDH levels were measurd by enzymic method in246cases with myeloproliferative neoplasma when initial diagnosis, serum NSE levels in113cases by electrochemiluminescence, and the results were statistically analyzed.RESULTS The serum LDH level for96.20%-98.21%patients were higher than normal, the mean was significantly higher than the control group (P<0.01), which of the mean is the highest (645.55±364.04) IU/L in PMF group. The levels of serum NSE for90.32%-96.67%patients were higher than normal, the mean was significantly higher than the control group (P<0.01), which of the mean is the highest (69.73±49.10) ng/ml in PV group. Serum LDH and NSE levels:They were positively correlated with hemoglobin (P=0.001, P=0.007) in PV group; They were positively correlated with platelet count (P=0.000, P=0.005) in ET group; They were negatively correlated with hemoglobin (P=0.019, P=0.022) and positively correlated with platelet count and white blood cell count OP=0.003,P=.016)(P=0.003, P=0.000) in PMF group. Serum LDH and NSE levels after treatment were significantly lower than before treatment with each group (P <0.01). Serum LDH and NSE levels were positively correlated (P=0.021,0.002,0.033) in each group.CONCLUSION The serum LDH and NSE levels of PV, ET, PMF were high and can reflect the degree of proliferation of blood cells and the dynamic monitoring of serum LDH and NSE levels help determine its efficacy. OBJECTIVE Neuron-specific enolase (NSE) was mainly expressed in neurons and neuroendocrine cells. In recent years, scholars study and put forward that the NSE is not a neuron-specific biomarkers. Pre-clinical data showed that NSE expression was elevated in polycythemia vera (PV), essential thrombocythemia (ET), primary myelofibrosis (PMF). K562and K562/A02cells are derived from human chronic myeloid leukemia (CML) red and white variable cell lines, CML is a myeloproliferative neoplasma category. By observing the expression of NSE in human leukemia cell line K562and its multidrug-resistant cell line K562/A02, the study explore the significance of the NSE in leukemia.METHODS ELISA was used to test the NSE expression in K562and K562/A02cells culture supernatant, flow cytometry detect NSE expression in K562and K562/A02cells, using the Western Blot technology further analysis of NSE expression in the cells.RESULTS ELISA results showed that K562and K562/A02cells can express NSE, NSE levels in K562and K562/A02cells culture supernatant than serum NSE levels in healthy subjects body was significantly increased (P<0.01). NSE levels in K562/A02cells compared with K562cells was significantly increased,(P<0.05). Flow cytometry and Western blot techniques showed expression of NSE in the K562and K562/A02cells, in multi-drug resistant cell line K562/A02NSE expression was significantly higher than that of K562, the difference was statistically significant (P<0.01).CONCLUSION Human leukemia cell line K562and its multidrug-resistant cell line K562/A02can both expression and secretion of NSE, while higher expression and secretion in multidrug-resistant cell line K562/A02may be involved in the resistance process of P-gp metabolic.