Changes And Significance Of Neuron-specific Enolase In Cerebrospinal Fluid Of Patients With Acute Leukemia During Treatment

Objective:To study the trauma for central nervous system(CNS) from CNS leukemia(CNSL) and intrathecal injection(IT) with methotrexate,arabinosylcytosin and dexamethasone through detecting neuron-specific enolase(NSE) content in cerebrospinal fluid(CSF).Methods:10 cases of acute non-lymphoblastic leukemia(ANLL),23 cases acute lymphoblastic leukemia(ALL) and 5 cases of CNSL patients were studied from 2004 to 2006.The NSE in CSF was detected with electrochemistry luminescence;and theβ₂-microglobulin(β₂-MG) was detected with radioimmunoassay.Result: NSE was(11.56±4.52ng/ml) in CNSL and there was not significantly higher than acute leukemia(AL) group(9.59±3.42ng/ml).There was no significantly change of NSE(9.96±3.37ng/ml) before IT,and (11.15±3.49ng/ml) at day 10,and(12.32±3.55ng/ml) at day 20,and(10.76±2.76 ng/ml) at day 56 after IT.β₂-MG was(5.42±1.1mg/l) in CNSL and significantly higher than AL group(1.67±0.21mg/l).Conclusion:IT with methotrexate,arabinosylcytosin and dexamethasone was safe and effective because of non-harm to CNS in the near future.β₂-MG in CSFwas a useful marker in diagnosis of CNSL,but NSE was not.