Effects Of Mitochondrial Calcium Unidirectional Transporter On Mitochondrial Division In Cerebral Ischemia / Reperfusion Injury In Rats

Objective To investigate whether mitochondrial calcium uniporter has effects on mitochondrial fission on the model of cerebral ischemia/reperfusion injury in rats.Methods The model was prepared by thread occlusion of middle cerebral artery for2hours, and followed by reperfusion for24hours. Eighty rats were randomly divided into4groups:A:sham-operated; B:Ruthenium red+ischemia/reperfusion; C:spermine+ischemia/reperfusion; D: ischemia/reperfusion. After24hours reperfusion, neuropsychological score, the mitochondrial free calcium content, the CN enzyme activities, Drp1expression and mitochondrial ultrastructure were observed above all the groups.Results Compared with group D, Ruthenium red preconditioning in group B could improve neuropsychological score, decrease the mitochondrial free calcium content, CN enzyme activities and Drp1expression, and improve the mitochondrial ultrastructure. While group C got the opposite results.Conclusions The activity of mitochondrial calcium uniporter has effects on mitochondrial fission this relationship between MCU and mitochondrial fission may possibly provide protection against ischemia/reperfusion injury.