Inhibitory Effect Of Honeysuckle Decoction On Staphylococcus Epidermidis Biofilm In

Objective:1,observation by Staphylococcus epidermidis biofilm formation and bacterial growth stateand growth in a laboratory environment, the choice of strains growing well as experimentalstrains.2, preparation honeysuckle decoction liquid will fold dilution effect on Staphylococcusepidermidis biofilm measurement honeysuckle decoction of Staphylococcus epidermidisbiofilm minimum inhibitory concentration (minimal inhibitory concentration, MIC), andobserve the different impact on the concentration of liquid adhesion of bacterial biofilms.3, with different concentrations of honeysuckle decoction different intervention levels ofbacterial biofilm growth model and influence by optical microscopy and scanning electronmicroscopy to observe their morphology.Methods:1, with Congo red plate isolated on statutory film production clinical isolates ofStaphylococcus epidermidis, and seeded in96-well microtiter plate was measured using amicroplate reader at a different time value A determines the amount of change in the growthof bacteria; with adhesion was measured in the same manner as in the96-well plate biofilmgrowth changes.2, with a decoction prepared crude drug concentration of honeysuckle decoction1g/lmL.3, biofilms with honeysuckle decoction fold dilution effect on the formation inStaphylococcus epidermidis, with the negative control group to determine the minimuminhibitory concentration.4, the role of the decoction with honeysuckle staphylococcal biofilms of differentconcentrations of the drug in different epidermal growth state of adhesion was determined on96-well plates with a microplate reader amount of surviving bacteria to determine theireffects on bacterial adherence.5, in order to make it grow as the carrier slides bacterial biofilms, with honeysuckledecoction of its role and erythromycin, respectively, compared to observed changes inbacterial biofilm morphologyResults:1, in the process of cultivating visible colony gradually increase of Congo red plate,3d afterthe formation of biofilm by staphylococcus epidermidis. Bacteria growth statistics resultsshow that with the increasing of incubation time, the concentration of the epidermisstaphylococcus aureus in TSB is on the rise, but adhesion in96-well plates on the surface of the bacteria or the formation of biofilm from4h after began to appear, as the extension oftime, did not change significantly the attached amount.2, as gold and silver hydrosols increasing solution concentration, the inhibition of epidermisstaphylococcus aureus biofilms gradually enhanced, gold and silver floral water decoction ofepidermis staphylococcus aureus biofilm MIC (minimum inhibitory concentration) of125mg/ml, when the concentration of250mg/ml, the bacteriostatic action of silver and goldhydrosols decoction is not increased, positive control group of epidermis staphylococcusaureus inhibitory effect is stronger.3, compared with negative control hole,1000,100mg/ml concentration of silver and goldhydrosols decoction on biofilm formation by staphylococcus epidermidis (1h,4h) in theearly years of the adhesion strong inhibition, the mature biofilm (8h) adhesion inhibition;100mg/ml concentration of silver and gold hydrosols decoction on epidermisstaphylococcus aureus biofilm inhibition is small; Positive control group of erythromycin onepidermis staphylococcus aureus biofilm inhibition is stronger.4, for the growth of3days of epidermis staphylococcus aureus biofilm, with250mg/ml ofsilver and gold hydrosols decoction on its role, it found that: after6h, adhesion of reducedby staphylococcus epidermidis and biofilm has no obvious changes (FIG.3-B1);1d,adhesion quantity decreased significantly by staphylococcus epidermidis and bacterialbiofilm damage (FIG.3-B2); Part3d, the adhesion of bacterial biofilm free, biologicalmembrane surface was designed in (FIG.3-B3);7d, the structure of the bacterial biofilm isdestroyed, biofilm is a large number of free, only a small amount of patch shape shadow onglass slide.Conclusion:1, according to the determination of the epidermis staphylococcus aureus biofilm growthshows that4h is bacteria adhesion and biofilm formation time to start, bacterial adhesion isincreased with the extension of time may be associated with96orifice plate at the bottom ofthe limited area.2, the experimental results show that gold and silver floral water decoction of epidermisstaphylococcus aureus biofilm MIC (minimum inhibitory concentration) of125mg/ml, andalso confirm the positive group does the epidermis staphylococcus aureus inhibitory effect oferythromycin is stronger.3, high concentrations of gold and silver floral water decoction can inhibit the bacteria in theearly years of the biofilm formation by staphylococcus epidermidis adhesion. Bacterialbiofilm of mature, this effect.4,250mg/ml of silver and gold hydrosols decoction can effectively remove bacteria biofilm,but the effect is better than erythromycin.