Effects Of Oxythiamine On Proliferation And Apoptosis Of Human Lung Adenocarcinoma Cell Line A - 549

Objective:The purposes of the study are to observe the effects of oxythiamine in lung cancer cell line A549in vitro and to explore the possible mechaniasm.Method:1.The morphological changes of the cells treatmented by oxythiamine were observed by using phase microscope.2.The inhibition of proliferation in vitro was measured by CCK-8assay.3.The flow cytometry indicated that oxythiamine also induced apoptosis of lung cancer cell line A549in vitro.Results:1. The growth of lung cancer cell line A549was significantly inhibited by the addition of oxythiamine in a concentration-dependent manner over24h of culturing. Gradually dose dependent morphology changes were shot under the light microscope.2.As the CCK-8assay demonstrated that oxythiamine could significantly inhibit the growth and proliferation of A549cells and the inhibiting effects had the dose-dependence and time-dependence.The maximum response was obtained with200umol/l of oxythiamine treated over48h.3.The flow cytometry was further used to identify the types of cell death. As shown in Figure2-2,2-4,2-6, oxythiamine showed a dose-dependent apoptosis, including early as well as late apoptotic cell death. The mean percentage of apoptotic cell death was about (22.977±1.333),(27.320±3.610) and (31.447±2.566)%in cells treated with2,20and200μmol/l oxythiamine by48h,respectively (P<0.01). In contrast, the cells that had not been treated with oxythiamine demonstrated normal cell viability without abnormal cell death.It suggests that oxythiamine can inhibit human lung adenocarcinoma A-549cells proliferation resulted in induced cell apoptosis.Conclusion:The exogenous addition of oxythiamine has an anti-tumour activity on lung cancer cell A549in a concentration-dependent manner.After oxythiamine cultured, visible apoptotic peak has been found.This suggests that the mechanism of the antiproliferative effect of oxythiamine may induce cancer cell apoptosis.