Study On Monophobe Proton Spectroscopy Of Skeletal Muscle Depression

PART ONE Application of proton magnetic resonance spectroscopy (1H-MRS) in skeletal muscle of healthy people[ABSTRACT] Purpose:In trying to evaluate the distribution and spectrum of metabolites of skeletal muscle in vivo with the use of Single-voxel proton MR spectroscopy. Materials and methods:Collecting16asymptomatic volunteers(n=16)of age from22to54years which including9males and7females. The mean age is28±9.29years. All the volunteers are tested by the same methods that we get the routine anatomic MRI pictures of the two palms first, then test the target muscle of the two palms respectively with the application of single-voxel1H-MRS. MR device in this study is Siemens3. OT VERIO imaging system, and the localization is assessed by a STEAM (stimulated echo acquisition mode) sequence。Getting the final data by processing original spectroscopy curve of1H-MRS with the unique SIEMENS software automatically. Results:The waves of single-voxel1H-MRS include intramyocellular lipids(IMCL,1.19ppm)、extramyocellular lipids(EMCL,1.53ppm)、choline(Cho,3.2ppm)and creatine(Cr,3.03ppm). we found that creatine has no significative statistical difference between two hands but opposite in gender. There is no cignificative statistical difference in content and relative content of intramyocellular lipids (IMCL) between female and male, but extramyocellular lipids(EMCL) content and its relative content have statistical difference in gender. The content and relative content of intramyocellular lipids(IMCL) between two hands are parallel in statistics and negative correlation exists between body mass index(BMI) and intramyocellular lipids(IMCL). Conclusions:1H-MRS is usefull to evaluate the metabolites of skeletal muscle in vivo and sensitive to small fractions of lipids inside myocytes (intramyocellular lipids; IMCL) and fatty septa between the muscle bundles (extramyocellular lipids; EMCL). PART TWO Application of proton magnetic resonance spectroscopy (1H-MRS) in healthy people and patients with denervated skeletal muscle injury to evaluate the value of1H-MRS.[ABSTRACT] Purpose:In trying to evaluate the distribution and spectrum of metabolites of skeletal muscle in vivo with the use of Single-voxel proton MR spectroscopy. Materials and methods:Collecting16asymptomatic volunteers(n=16)of age from22to54years (including9males and7females) and19cases(n=19) of deffrent degrees of injury of the ulnar nerve(including3females and16males)which aged from16to57years. The mean age of the volunteers and patients are28±9.29years and34Q14yearsAll the volunteers and patients are tested by the same methods that we get the routine anatomic MRI pictures of the two palms first, then test the target muscle of the two palms respectively with the application of single-voxel proton MR spectroscopy. MR device in this study is Siemens3. OT VERIO imaging system, and the localization is assessed by a STEAM (stimulated echo acquisition mode) sequence。 Getting the final data by processing original spectroscopy curve of1H-MRS with the unique SIEMENS software automatically. Results:we found that the content and relative content of intramyocellular lipids(IMCL) and extramyocellular lipids(EMCL) have statistical differences between injured side and healthy side (P<0.01). Especially, the deffrence of IMCL between injured side and healthy side is obvious than EMCL (P<0.01). The content of creatine has statistical raise between two sides (P<0.01). And the same results were found between patients and volunteers as above. And there are the same statistical deffrences between injured group and volunteer group. In addition, more severe injury, intramyocellular lipids(IMCL) and extramyocellular lipids(EMCL) content increased significantly. Intramyocellular lipids(IMCL) and extramyocellular lipids(EMCL) content and the time of injury were positively correlated, in which, the extracellular lipid content increased with the time of injury directly. Conclusions:Single voxel Proton magnetic resonance spectroscopy1H-MRS technology can be used to distinguish the IMCL and EMCL. At the same time,1H-MRS detected that IMCL raises early in nerve injury cases, so IMCL has important diagnostic value in early phase of denervated injury. And EMCL has long-term diagnosis and dynamic monitoring value of denervated skeletal muscle injury.