| Section 1.Objective To investigate the pathogenesis and immune response of the syphilis rabbits model under the different inoculation methods and to provide the further evidence for the establishment of the pharmacodynamics evaluation model. Methods Rabbits were randomly divided into the testes inoculation group and back-intracutaneous inoculation group with 10 rabbits in each group.The rabbits offrom the testes inoculation group were inoculated 2X107T. pallidum Nichols strains in total and the back of the rabbits from the back-intracutaneous inoculation group were shaved and six spots were chose to be inoculated 2X107T. pallidum Nichols strains in total too. The clinical manifestations and the serologic reactions were observed Results The most obvious clinical symptoms of the testes inoculation group can be observed on the day (14.50±1.08) and that of the back-intracutaneous inoculation group can be observed on the day (29.00±10.30). The difference was statistically significant(P<0.05). The highest RPR titer of the two groups was measured by rank sum test. The difference was statistically significant (P<0.05). The RPR titers of the testes inoculation group were higher than that of back-intracutaneous inoculation group. Conclusions The pathogenesis and immune response of the syphilis rabbits model under the different inoculation methods were distinct.The RPR of the testes inoculation group turned positive earlier and the titers were higher. The symptoms of the back-intracutaneous inoculation group were similar to the human primary syphilis chancre.Section 2.Objective Based on the result of the first part,to investigate the efficacy of ceftriaxone in the treatment of syphilis, find out the optimal experimental conditions, the detection index and the experimental drug dose for the subsequent pharmacodynamic evaluation experiments of syphilis drugs to provide a theoretical basis. Methods There were 18 normal rabbits in this experiment, one of them was randomly selected as negative control group.The rabbit was marked six points on the shaved back. Each point were injected saline solution into the epidermis. Another 17 rabbits were injected Tp into the six marked points on the epidermis. When the serum RPR titers of the 70% rabbits remain the same number for the consecutive 3 weeks, according to the grading of rabbit skin lesion, the rabbits of each group were randomly divided into procaine penicillin treatment group, ceftriaxone treatment group and untreated group, and respectively had 8, 8 and 1 rabbit. The rabbits of two treated group randomly divided into four sub-groups, each group was continuously treated for 10 days. The clinical manifestations and the serologic reactions were observed. Results At the end of the experiment, procaine penicillin and ceftriaxone treatment group rabbits respectively gain the weight (0.49 ±0.31) Kg and (0.41 ± 0.18) Kg.Two independent sample t-test showed t= 0.609, P= 0.55> 0.05, there was no statistically significant difference. Fisher’s exact probability method is used to test the rabbit serum negative rate of two groups, there was no statistically significant difference P= 0.315> 0.05. So it could not be thought that the serum negative rate of penicillin treatment group and ceftriaxone group had difference. There were 2 rabbits of high dose group in the procaine penicillin group showed serum negative, low dose group had failed to turn negative. There were 3 rabbits of high dose group in the ceftriaxone group showed serum negative, two rabbits of low dose group showed serum negative. The two treated groups all showed that high dose group curative effect was better than the low dose group. Conclusions Ceftriaxone can be effective to treat syphilis. The symptoms of the back-intracutaneous inoculation group were similar to the human primary syphilis chancre. However, because of the low incidence of skin lesions, slight change of serological reaction, the low RPR drop degree. And the observation of skin lesions was hard before and after the treatment.Therefore, it still need to be compared with the method of testes inoculation, to draw a conclusion that whether the method of back-intracutaneous inoculation was suitable as the syphilis pharmacodynamic model. |
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