| In traditional Chinese medicine is thousands of years of Chinese development and perfect description, its characteristic is that the overall concept Theory of TCM (Traditional Chinese Medicine)syndrome differentiation and treatment, etc., these are based on meridian theory. Meridian system inside and outside the human body contact as a unified whole segments of the viscera, and through the rows in the grid of moisten the zang-fu organs inside and outside, therefore, the body health can be expressed through the meridians and acupoints.Acupuncture is by stimulating the corresponding acupuncture points to restore the body in order to achieve dynamic balance, eventually attain the goal of treatment. Acupuncture is divided into two methods of acupuncture and moxibustion, each have expertise, combined with the actual condition or independence or unification.Ling shu records "If there is no effect, acupuncture moxibustion can be used", moxibustion is the necessary supplement for acupuncture moxibustion therapy, and even in some of other disease treatment effect of incomparable advantage.Curative effect of moxibustion and acupuncture as well as problems in time and the amount of stimulus, they are the main factors influencing the curative effect. Moxibustion intervention time is relative to the disease process, including disease before intervention and disease development process, belongs to the early intervention; moxibustion stimulating amount in moxa cone size, time, etc. This experiment mainly by observing the different intervention time and amount of stimulus of moxibustion, discusses different moxibustion methods on haemorrheological nature card type dysmenorrhea treatment effect in rats.Objectives:Combining model preparation of cold stagnation syndrome with dysmenorrheal together, successfully set up dysmenorrheal rat models of cold stagnation syndrome in the early related experiments by us. On the basis of observation of different intervention time moxibustion close Guanyuan of acupuncture point on dysmenorrheal rat models uterus microcirculation, prostaglandin receptor FP,EP2,FP/EP2. Further compare moxibustion on dysmenorrhea occurred early in the process of interventional therapy with dysmenorrhea model after simple treatment effect.Methods:64 three-month-old female SD rats during anestrus were divided randomly into the saline group, The cold stagnation dysmenorrheal model group, A group, B group,16 in each group (8 to measurement of uterus microcirculation used in experiments A, The other 8 used in experiment B, to test the uterus tissue PG receptors,). Specific building method:Whole body freezing method combined with estradiol benzoate injection to set up model,Estradiol Benzoate injection was administrated fixed time every day to animals of dysmenorrheal model group, A group, B group by subcutaneous injection. The injection continued for 10 days with the dosages ranged from 0.5mg/each animal in the first day to 0.2 mg/each from the second to the ninth day, and to 0.5mg/each animal in the last day.1 hour later after the last time of Estradiol Benzoate injection, oxytocinum was given to animals with pharmaceutical intervention group (2U/each animal) by intra-peritoneal injection the dysmenorrheal model group, A group, B group.and 1-5 days after the injection, was placed into -25℃ freezer for 4 hours (with a 5 seconds interval between every 2 hours for ventilation). Animals in the saline group were injected with saline of the same quantum.Moxibustion different intervention methods are as follows: A group (model is established after the intervention):The 10th day after the injection of oxytocin, Treatment at a time by moxibustion, that is to say after the dysmenorrhea model is successfully established to treat. B group (model before the intervention):Treatment at a time by moxibustion after the injection of liquid medicine,on the 6th,8th,10th day.6th,8th days before the dysmenorrhea treatment,10th day after the dysmenorrhea treatment.Results:1. Effects of moxibustion early intervention on dysmenorrheal rat models of cold stagnation syndrome uterine blood vessel diameter, number, color, clarity.Compared with that of the saline group, rats in the cold stagnation dysmenorrheal group had remarkably shrunken uterus micro-vessel diameter and definition at all time points from the 5th to the 60th minutes (P<0.01). They also had significantly reduced uterus capillary diameter, numbers and micro-vessel color (P<0.01,P<0.05) from the 5th to the 60th minute.In contrast with the cold stagnation dysmenorrheal group, rats in the A group had significantly higher uterus micro-vessel and capillary diameter at all the time points from the 5th to the 60th minute (P<0.01,P<0.05), significantly increased uterus capillary numbers at all the time points from the 5th to the 60th minute (P<0.01), significantly increased uterus micro-vessel numbers at all the time points from the 40th to the 60th minute (P<0.01,P<0.05). The definition of uterus micro-vessels of animals in the group were obviously improved at all the time points from the 5th to the 60th minute (P<0.01), significantly improved micro-vessel color at all the time points from the 5th to the 20th minute (P<0.01).In contrast with the cold stagnation dysmenorrheal group, rats in the B group had significantly higher uterus micro-vessel and capillary diameter at all the time points from the 5th to the 60th minute (P<0.01), significantly increased uterus capillary numbers at all the time points from the 5th to the 60th minute (P<0.01), significantly increased uterus micro-vessel numbers at all the time points from the 40th to the 60th minute (P<0.01). The definition of uterus micro-vessels of animals in the group were obviously improved at all the time points from the 5th to the 60th minute (P<0.01,P<0.05), significantly improved micro-vessel color at all the time points from the 5th to the 30th minute (P<0.01,P<0.05).In comparison with the A group, the uterus micro-vessel and capillary diameter or numbers-the color and definition of uterus micro-vessels of animals in the B group were improved at all the time points from the 5th to the 60th minute.But there was no significant difference among this indexes.In contrast with the cold stagnation dysmenorrheal group,A group set of indicators of P value than group B group.although there was no significant difference among this indexes, the curative effect of group B was superior to group A trend.2. Effects of Moxibustion early intervention on dysmenorrheal rat models of cold stagnation syndrome uterine prostaglandin receptor FP,EP2,FP/EP2.Compared with that of the saline group, The cold stagnation dysmenorrheal group PGF2 a receptor content of model group increased significantly (P<0.01), rised EP2 content but no statistical difference,significantly increased in PGF2 a receptor/EP2 (P<0.01).In contrast with the cold stagnation dysmenorrheal group, rats in the A group had significantly decreased PGF2 a receptor, EP2 and PGF2 a receptor/EP2 (P<0.01). Group B was the same situation.In comparison with the A group, rats in the B group had decreased PGF2 a receptor, EP2 and PGF2 a receptor/EP2 (P<0.01), but no statistical difference.Conclusions:1.Compared with that of the saline group, rats in the cold stagnation dysmenorrheal group set of indicators in uterus microcirculation had remarkably shrunken. Intrauterine environment imbalances,lead the uterus tissue presents cold stagnation nature symptoms.2. After the action of moxibustion close yuan of acupuncture point, rats in the cold stagnation dysmenorrheal group the uterus microcirculation status markedly improved, prostaglandins in uterine tissue related receptors geted right, environment back into balance within the body3. Have different curative effect by different intervention time moxibustion close Guanyuan of acupuncture point on the cold stagnation dysmenorrheal models, in comparison, from the trend of early intervention in the process of dysmenorrhea the effect of moxibustion, there are better than that of dysmenorrhea after treat. |
