Extraction Of Polysaccharide From Cordyceps Militaris And Its Protective Effect On Alcohol - Induced Liver Injury

Cordyceps Militaris polysaccharides are important biologically active substances. The researchers found that Cordyceps polysaccharide has many physiological functions, such as antioxidant, anti-tumor, enhancing immunity, reducing blood pressure and protecting liver and so on. In this study, Cordyceps militaris culture medium as material, different extraction methods were used to extract Cordyceps polysaccharide, and discusses the affect of polysaccharide matrix of yield, viscosity and in vitro antioxidant activity with different extraction methods.A more comprehensive comparison of various advantages and disadvantages of the methods, and its acute and subacute effects of alcohol-induced liver injury prevention research. This study will help to enhance the added value of cordyceps militaris stroma polysaccharides, and provide theoretical support for cordyceps militaris stroma polysaccharides functions as an antioxidant products.Six different extraction methods, including water extraction, enzymatic extraction, ultrasonic method, ultrasonic-enzymatic method, microwave method and microwave-enzyme method, were used to extract Cordyceps polysaccharide from Cordyceps militaris culture medium. The extraction yield, viscosity and the impact of antioxidation were evaluated to compare the advantage and disadvantage of different extraction methods. The results showed that polysaccharide prepared by ultrasonic-enzymatic extraction had the highest extraction yield 30.27%, while water extraction had the lowest yield 10.69% at the same time.Polysaccharide viscosity with the increase of polysaccharide solution concentration by different extraction methods, and the apparent viscosity at the same concentration was:water extraction> microwave extraction> enzyme extraction> microwave-enzymatic extraction> ultrasonic extraction> ultrasonic-enzymatic extraction. The polysaccharide prepared by ultrasonic-enzymatic extraction exhibited the strongest antioxidant activity, while water extraction showed the lowest antioxidant activity.To explore the protective effect of cordyceps militaris stroma polysaccharides(CMSP) on alcohol-induced acute liver injury in mice. Mice were randomLy divided into blank control, alcohol-induced liver injury model, positive control and CMSP treatment (at doses of 150,300 and 600 mg/(kg·d))groups. After 30 consecutive days of intragastric administration, all the mice except for blank control group were given by gavage 50% alcohol at a dose of 12 mL/kg to induce acute liver injury. After 12 h alcohol administration, all the mice in each group were killed by cervical dislocation and blood were collected for the measurement of Superoxide dismutase (SOD) activity, Triglyceride (TG) content, alanine transaminase (ALT) and aspartate transaminase (AST) levers in serum, the liver, spleen and thymus were collected to calculate the coefficients, preparation of liver homogenate were measured the activities of Superoxide dismutase (SOD) activity, Glutathione peroxidase(GSH-PX) activity, Malondialdehyde(MDA) and glutathione(GSH) content, and pathological observation of liver tissue. The results showed that the liver, spleen and thymus coefficients, serum SOD activity TG content, ALT and AST activities, and SOD activity, GSH-PX activity, MDA and GSH content in the liver of mice from the CMSP groups were significantly different to those of the liver injury control group (P<0.05or P< 0.01). The conclusion is showed as following:CMSP has significantly hepatoprotective effect on alcohol-induced acute liver injury in mice.In addition, we further explore the effect of Cordyceps militaris stroma polysaccharides(CMSP) on alcohol-induced sub-acute liver injury in male mice. Methods Mice were randomLy divided into blank control, alcohol-induced liver injury model, positive control and CMSP treatment (at doses of 150,300 and 600 mg/(kg·d))groups. Animals were administered orally with 30% alcohol at 6 h after the doses of CMSP everyday for twenty-eight consecutive days except mice in normal group. All animals were sacrificed, and blood were collected for the measurement of glucose(GLU)content, total bilirubin(TBIL)content, alanine transaminase(ALT) activity, aspartate transaminase(AST)activity, alkaline phosphatase(ALP)acitivty, triglyceride(TG)content, total cholesterol(CHOL)content, and lactic acid dehydrogenase(LDH) activity in serum, the livers were collected to calculate the coefficients, preparation of liver homogenate were measured the activities of alcohol dehydrogenase (ADH) activity, Glutathione peroxidase(GSH-PX)activity, Malondialdehyde(MDA) and glutathione(GSH) content, and pathological observation of liver tissue. The results showed that the liver coefficients, serum GLU, TBIL, TG and CHOL contents, and ALT, AST, ALP and LDH activities, and ADH activity, GSH-PX activity, MDA and GSH contents in the liver of mice from the CMSP groups were significantly different to those of the liver injury model group (P<0.05 or P< 0.01). The conclusion is showed as following:CMSP has significantly hepatoprotective effect on alcohol-induced sub-acute liver injury in mice.