Controllable Synthesis, Assembling Of MTX Nanocomposites And Its Inhibitory Effect On Tumor Cells

Nanoparticles with uniform size and controlled morphology are received more and more attention in the area of nanotechnology. Actually, it is a very important motivation for the development of nanoscience. In the burgeoning nano biomedicine field, the study of the drug carrier get a widely attention because of its unique charm In this paper, Methotrexate (MTX) is choose as the guest drug molecule, a series of MTX/nanocarrier are synthesized. The structure, property and inhibition towards A549 cells are studied, details are summarized as following:1. The synthesis of Au-MTX@SiO2 was carried out following a modified Stober method. Furthermore, effects of Au and MTX is discussed and the result shows the core-shell structure have a important influence for drug loading. In addition, the morphological evolution is also studied in PBS (pH=7.4) at 37 ℃. For the in vitro bioassay, obvious cancer cell inhibition can be achieved in the presence of a low concentration of Au-MTX@SiO2 NPs and the efficacy can be also enhanced by irradiation of Au core.2. Using Au-MTX@SiO2 as template, a novel kind of Au yolk/LDH shell nanoparticles are obtained. The structure and synthesized condition of the nanoparticles are discussed and the result shows that the hollow structure is held by the combination between LDH sheet and SiO2. At last, the in vitro cytotoxicity is examined and the result indicates that the yolk/shell nanovehicles loaded with MTX exhibit superior anticancer efficacy, comparing to the free drug in MTT assay.3. The synthesized MTX/HAP nanoparticles are studied by a manner of the effect of PEG and MTX. Then the mechanism is explained. Meanwhile, the drug release and cell inhibition are also studied. In vitro cytotoxicity is examined and the result indicates that the MTX/HAP exhibit superior anticancer efficacy, comparing to the free drug MTX in MTT assay.