Study On The Mechanism Of Reducing Proteinuria In Compound Snake Dragon Capsule Based On The Best Compatibility Of Effective

Objective: This topic through the observation of the best compatibility of compound She Long capsule before and after treatment of adriamycin induced nephropathy rats,Changes of rat glomerular ultrastructure and the expression of glomerular podocyte marker Nephrin and Podocin protein and m RNA, investigating the the mechanism of reducing urinary protein about compound She Long capsule of effective parts of the best compatibility.Methods: Choose 90 healthy male clean grade SD rats, adaptive feeding for one week,randomly selected 10 rats as control group, copy the rest of rats to adriamycin nephropathy model Put the Adriamycin nephropathy model rats to model group, positive group, Compound She Long Capsule group of low, medium and high. 11 in each group, Continuous irrigation for 8weeks.After irrigating, watch the effect of Compound She Long Capsule to 24 h UTP,ALB,TP,BUN,Cr,CHO,TG,TNF-α,and IL-8 of rats; SP was used to observe the effection of the expression of Nephrin,VEGF,HSPG about rat kidney tissue; Rat kidney podocyte slit on the expression of Nephrin protein and Podocin protein and its m RNA aperture diaphragm by using Western blot and Real-time PCR. Observation the effect of Compound She Long Capsulechanges’ histological change of ratglomerular podocyte ultrastructure pathology by using renal tissue pathology technique.Result: Compound SheLong Capsule can induce nephropathy the 24 h UTP in rats, and the serum albumin and serum lipid levels were improved, and the renal protective effect was significant.Research on Mechanism of action shows that The contents of IL-8 and TNF- in the serum of rats with adriamycin induced nephropathy were lower., And it was confirmed by protein and gene levels that the Compound She Long Capsule can significantly improve the renal tissue of the rats with adriamycin nephropathy in nephrin, HSPG, podocin expression and the down-regulation of VEGF expression levels, maintain the integrity of the podocyte morphology and function, thereby reducing the leakage of urine protein.Renal pathological morphology and structure of the research show that The capsule can lighten the pathological damage degree of rat glomerularglomerular nephropathy model.Conclusion: Compound She Long Capsule can proteinuria of rats with adriamycin nephropathy by up regulating the expression of Nephrin protein, Podocin protein and m RNA in the glomerular pore cell of rats with adriamycin induced nephropathy and improve the pathological changes of the ultrastructure of the renal glomerular.