Effect Of Metformin On The Sensitivity Of Tamoxifen To MCF-7 Cells

Purpose：Research on effect tamoxifen and metforminon breast cancer cells MCF-7Tostudy the impact of tamoxifen on breast cancer cells combined with metformin in MCF-7andexplore the mechanisms of metformin combined with tamoxifen of tumor inhibition.Material and method：1.The effect of different concentrations of metformin and metformin combined withtamoxifen on MCF-7cells and MTT assay measuring section48and72hours of OD values,inhibition rate was calculated for each group;2.protein expression levels using the Western blot technique to detect cell, using the Real timePCR technology to detect cell mRNA levels;3.Conduct experiments to observe the migration of cells scratches.Results：MTT results showed that metformin on MCF-7cells was inhibited with increasingconcentrations of metformin and the extension of time, the inhibitory effect more obviousresults among groups with significant differences (p <0.05). The study also found thatmetformin with tamoxifen alone when compared with combined inhibitory effect ofmetformin significantly (p <0.05), and also showed a dose-dependent relationship. WesternBlot and RT-PCR results showed that: metformin and (or) after tamoxifen treatment48hours,HIF-1α-1, mTOR, Vimentin3expression level of protein and mRNA were decreased, withincreasing dose of metformin expression decline, this phenomenon is more evident in thecombination group, the difference was statistically significant. Scratch experimental resultsshow that the healing of metformin on tumor cells inhibited their combination can inhibittumor migration. Conclusion：1.Metformin inhibits MCF-7cell proliferation and induceapoptosis, and with the increase ofthe concentration,the effect will be more conspicuous;2.Metformin can inhibits MCF-7cells epithelial-mesenchymal-transition effects;3.Combined metformin with tamoxifen to intervene MCF-7cell has a synergistic effect toproliferation inhibition,metformin can Improve the sensitivity of endocrine therapy.