Expression Of FHL2 In Hepatocellular Carcinoma And Its Significance

Backgroud＆aim: FHL2is a LIM-only protein that belongs to the Four-and-a-HalfLIM-only protein family. It consists of four LIM domains and one N-terminal halfLIM domain. It is strongly expressed in cardiac and skeletal muscle cells but a muchlower level was observed in other tissues and cell types.The LIM domains in thisprotein can function as an adapter or modifier in protein interactions．FHL2was firstidentified as being downregulated in human rhabdomyosarcoma cells, suggesting arole in tumor devel-opment。Many recent studies have reported the differ-entialexpression of FHL2in tumor tissues. The intriguing aspects of FHL2beingasoncoprotein or tumor suppressor may be related to its interaction with differentpartner proteins in different cell types. So far, the precise FHL2function in livercancer cells is still unclear.The research aim to detect FHL2expression in livercancer and cell lines.Methods:with the Western-Blot、Rreal-time、immunohistochemical,we detectFHL2expression in liver cancer and cell lines in different levels.Results:ln different levels,FHL2expression in liver cancer was lower comparedwith the adjacent tumor tissues. In live cancer cell lines．FHL2expression was weak.Immunohistochemiacal results shows that the FHL2are mainly located in thenucleus and cell membrane．Conclusion:1. Compared with the adjacent tumor tissues, FHL2expression was lower.2. In live cancer cell lines，FHL2expression was weak. Objective:To construct the recombinant lentiviral vector containing human FHL2gene and measure the expression level of FHL2in hepatocellular cell line of HUH-7. Methods:The FHL2fragment was amplified by PCR and subclonedinto the lentiviral vector pLV. The resultant lentivirus were confirmed by PCR, restriction enzyme digestion and DNA sequencing. Recombinant lentivirus (PLV-FHL2) were produced from293T by transient calcium-phosphate co-transfection withpWPT-FHL2, CMVR8.91and pCMV-VSV-G. HUH-7cells were infected by PLV-FHL2lentivirus, and the expression of FHL2was confirmed by Western blotting.[Results]:The recombinant lentiviral vector carried the FHL2gene was successfully constructed. Western blot analysis revealed that the FHL2gene can be correct transcripted and translated in HUH-7cell which infected by PLV-FHL2.[Conclusion]:The recombinant lentiviral vector pLV-FHL2was constructed successfully, it could be used to transfect hepatocellular carcinoma cells. FHL2could be stablely expressed. Backgroud&aim:Hepatic hemangioma is the most common benign liver, including cavernous hemangioma sclerosing hemangioma, hemangioendothelioma, and capillary hemangioma, hepatic cavernous hemangioma, the most common. The source of the liver hemangioma has two views, one is that the hemangioma is a vascular dysplasia formation. But more scholars believe that the hemangioma is a benign liver tumors. Metalloproteinase of extracellular matrix degrading enzymes, it is a zinc-dependent enzyme family. MMP-2matrix metalloproteinase (of MMPS) family members, studies have shown that in recent years and then tumor-mediated extracellular matrix degradation plays a key role, however, the basement membrane and matrix degradation of the angiogenesis necessary for endothelial cell migration is extremely important, so that the expression of MMP-2is a key factor in tumor angiogenesis. Clinical hepatic hemangiomas are more common in young women, reported during pregnancy or oral contraceptives hemangioma can be increased rapidly and the onset of symptoms. Most of the previous studies focused on the hepatic hemangioma is the compliance mechanism of the female hormone above. Of MMP-2a key role in the generated tumor angiogenesis mechanism, we have reason to believe that the metalloproteinase-2can promote angiogenesis, to promote the occurrence and development of hepatic cavernous hemangioma. This study was to investigate the expression and significance of matrix metalloproteinase MMP-2in hepatic hemangioma.Methods:1.Collect the lvier hemangioma and normal liver tissues. 2. with the Western-Blot、Rreal-time、immunohistochemical,we detect MMP-2expression in liver hemangioma and normal liver tissues in different levels.Results:1. MMP-2mRNA expression in Hepatic hemangioma group was significantly higher than the normal tissue group.2.MMP-2protein expression in Hepatic hemangioma and normal liver tissues:The trend of MMP-2protein expression is basically the same with MMP-2mRNA expression.3. Immunohistochemistry:light microscope, the expression of MMP-2positive cells were brown, positive particles was mainly localized in the cell membrane. MMP-2expression in liver hemangioma was higher than in liver normal tissues.Conclusion:MMP-2may play an important role on the pathogenesis of hepatic hemangioma.