Study On The Main Pharmacodynamics And Mechanism Of Evodiamine And Its Preparations In Treating Gout

Objective:Through studying the effect of evodiamine in reducing serum uric acid and mechanism’s preliminary discussion,and its preparation of evodiamine dispersible tablets’s acute toxicity safety evaluation and main pharmacodynamics research,to provide a theoretical basis for the clinical development of safety, effective, stable, economic gout drug.Methods:1)Through feeding chicken high protein and high calcium to build chicken model of hyperuricemia and observe the effects of evodiamine in reducing serum uric acid and xanthine oxidase (XOD), adenosine deaminase (ADA), guanine deaminase (GD), aspartate aminotransferase (AST), alanine aminotransferase (ALT) activity and urea value change in the serum.2)Through determining the maximum dosage in mice to observe the acute toxicity of evodiamine dispersible tablets.3)Through xylene-induced Pinna swelling modle on mice and sodium urate-induced acute paw edema model on rats,to observe the effects of evodiamine dispersible tablets on inflammation. Through the method of acetic acid induced mice writhing to observe the evodiamine dispersible tablets’s analgesic effects.Through intragastric administration of the rats hypoxanthine firstly and then injecting oxygen hydrochloride acid potassium salt subcutaneously to establish hyperuricemia model and observe the effect of evodiamine dispersible tablets in reducing serum uric acid.Results:1)Compared with the model group, in the administration of7d and14d,the low dose group of evodiamine could significantly reduce the content of UA in the chicken serum;in the administration of7d, the low dose group could significantly reduce the content of UA in the Chicken manure;in the administration of14d,the high and low dose group could significantly reduce the xanthine oxidase(XOD) activity in the chicken serum;in the administration of7d and14d,the low dose group could significantly reduce the adenosine deaminase (ADA)activity in the chicken serum;in the administration of7d and14d, the high and low dose group could significantly reduce the guanine deaminase (GD)activity in the chicken serum;in the administration of7d and14d,the aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activity and Urea value of high and low dose group both had no obvious change.2)After intragastric administration of evodiamine dispersible tablets to mice immediately and observed for14d,there was no one died and observed the main organ no abnormality was discovered and the maximum dosage is2216mg/kg(This dose express evodiamine’s dose).3)Compared with the control group, each dose group of evodiamine dispersible tablets had significantly inhibits to the Pinna swelling of mice and acute paw edema of rats; high dose group had significant inhibitory effects on the pain induced by acetic acid.Compared with the model group,each dose group of evodiamine dispersible tablets had significantly effects in reducing serum uric acid.Conclusion:1)Evodiamine has the effects of reducing serum uric a-cid,and preliminarily revealed that the mechanism may be associated with the decline of XOD,ADA and GD activity.2)Intragastric ad-ministration of evodiamine dispersible tablets to mice the maximum dosage is2216mg/kg(This dose express evodiamine’s dose),and there was no obvious toxic reaction and no one died,and shows that at the application of this dose is safe.3)Evodiamine dispersible tablets has the effects of anti-inflammatory,analgesia and reducing serum uric acid.