Effects Of Radiation And Chlorination On The Expression Of IER5 Gene In Cervical Carcinoma

Purpose：The purpose of this experiment was to express the effect relationshipbetween radiation dose and IER5in human cervical cancer gene, and effect ofnitidine chloride on the expression of IER5in human cervical carcinoma Helagene in cells, radiation sensitizing effect of nitidine chloride.Material and method：This study collected44cases of cervical cancertissues were taken from2011November to2012, December in Liaoning ProvincialTumor Hospital patients with cervical cancer tissue specimens. Includingpretreatment group,10Gy group,20Gy group, radiation radiation30Gy radiationgroup,40Gy group,50Gy group and radiation radiation radiation dose≥50Gyradiation group. Expression of IER5protein was detected by werstern blot. Atthe same time, human cervical carcinoma Hela cells in vitro as research object,with different concentration of nitidine chloride in Hela cells treated with24hours and48hours, treatment of Hela cells combined with radiation andnitidine chloride, detection of Hela cell growth inhibition by MTT method; Helafor the detection of cell apoptosis by flow cytometry, expression Hela cellprotein was detected by IER5werstern blot.Results：1Radiation on cervical cancer tissue IER5expression：Before treatment groupof gray value of0.87±0.15,10Gy radiation group gray value of0.81±0.13,20Gy radiation group gray value of1.37±0.19,30Gy radiation group gray valueof1.53±0.47,40Gy radiation group gray value of1.12±0.24,50Gy radiationgroup of gray value of0.95±0.83,>50Gy radiation group gray value of1.05±0.26.10Gy radiation group P=0.636>0.05compared with before treatment group,the difference was not statistically significant;20Gy radiation group,30Gygroup and40Gy group respectively radiation radiation treatment group compared to P<0.05, the difference was significant;50Gy radiation and>50Gy radiationgroup were compared with before treatment, P>0.05, the difference was notstatistically significant.30Gy P<0.05in the exposed group compared with othergroups, the difference was significant.2Effect of nitidine chloride on Hela cells2.1Proliferation was determined by MTT results showed: nitidine chloride caninhibit the proliferation of Hela cells, and a "time dose" certain dependence(P<0.05), nitidine chloride combined with radiation after the "time dosedependence" still exists (P<0.05) with statistical significance. Nitidinechloride combined with radiation group compared with the simple drug group, thetumor cell growth inhibition rate of the former than the latter, the differencewas statistically significant (P<0.05). Nitidine chloride in4μ g/ml groupwithin48hours after the inhibitory rate of47.7%nearly half inhibitory rate,analysis of MTT data and analysis of drawing out48hours of nitidine chlorideIC50was3.97418μ g/ml using ORIGIN50softwar.2.2Flow cytometry results: nitidine chloride can cause apoptosis in Hela cells,and a "time dose" certain dependence (P<0.05) combined with radiation, the "time-and dose dependence" still exist (P<0.05), the difference was statisticallysignificant. Nitidine chloride combined with radiation group compared with thesimple drug group, the apoptosis rate of the former is higher than the latterat the same time, the same concentration (P<0.05), the difference wasstatistically significant.2.3Western blot imprinting hybridization detection IER5protein expressionchange the result，4Gy radiation dose radiation group and control group,radiation group IER5expression amount is1.29times higher than that of controlgroup, which suggests that radiation can induce the Hela cell IER5expressionupregulation. Compared to the drug group and the control group, the expressionlevel of IER5is1.47times higher than that of control group, suggesting thatnitidine chloride could induce up regulation of IER5expression. Radiation combined with drug group compared with radiation group, the expression levelof IER5is1.21times the radiation group, the expression level of IER5is1.47times higher than that of control group.Conclusion：1. IER5gene in cervical carcinoma between the radiation dose of20~40Gy, upregulate the expression of IER5protein, in the radiation dose, IER5gene asthe radiation dose increased expression generally increased in the downwardtrend, and the30Gy radiation group IER5genes are upregulated in the mostobvious.2. Nitidine chloride could inhibit the growth of cervical carcinoma Hela cells,promote the apoptosis of tumor cells, and a "time dose" certain dependence(P<0.05); nitidine chloride combined with irradiation can inhibit the growthof cervical carcinoma Hela cells, promote the apoptosis of tumor cells, was "timedose" certain dependence (P<0.05). The mechanism may be related to increasedradiation sensitivity related gene IER5.