Effect Of Jiawei Tongfu Decoction On Nuclear Factor - KB Activity In Colonic Tissue Of Rats With Ulcerative Colitis

Purpose：Through experimental study on the effects of colon tissue soupflavored tong fu nuclear factor-k B activity (P50, P65protein) rat model ofulcerative colitis and protein expression levels, to further explore theirulcerative colitis effect, provide a definitive experimental evidence for themedical treatment of the disease.Material and method：70SD rats of clean grade were randomly divided intosulfanilamide pyridine group, the normal group, spleen intestinal bolus pillgroup, model group, Relaxing soup flavored high-dose group (32.4g/d), soupflavored tong fu dose group (10.8g/d), Relaxing soup flavored low-dose group(3.6g/kg). Selection of acid (TNBS)-Method ethanol, animal model of ulcerativecolitis, from the first four days of the start modeling the normal saline groupand model group, which are complementary to each group to spleen and intestinepill, sulfanilamide, Relaxing soup flavored high-dose, dose-flavored soupRelaxing, Relaxing soup flavored low-dose for21days were needed to death.Detection of colon tissue nuclear factor rat model of ulcerative colitis NF-kB (P50, P65) and protein expression levels.Results：1. Normal and model group, the activity of nuclear factor-kB (P50, P65protein)levels increased protein expression increased, there are significantdifferences (p <0.05).2. Nuclear factor-P50protein in normal and model group, Relaxing soup flavoredhigh, medium and low dose group, pill group, s sulfanilamide pyridine group P<0.05was considered statistically significant; flavored pass Fu soup high,medium and low dose group, spleen intestinal bolus pill group, sulfanilamidegroup compared with model group, nuclear factor-P50protein content decreased, it is notable difference (P <0.05); flavored Relaxing soup high dose group ofnuclear factor-P50protein content was significantly lower than spleenintestinal bolus pill group, a difference (P <0.05).3. Nuclear factor-P65protein in group and model group, soap flavored high, mediumand low dose group, pill group, comparing P <0.05was considered statisticallysignificant, compared with the sulfanilamide group P>0.05for the differencewas not statistically significant; flavored soup Relaxing high, medium and lowdose group, spleen intestinal bolus pill group, sulfanilamide group comparedwith model group, nuclear factor-P65protein content decreased, there weredifferences (P <0.05); flavored soup Relaxing high dose group of NF-P65proteinlevels were significantly lower than B spleen intestinal bolus pill group, adifference (P <0.05).Conclusion：1. Relaxing soup can reduce rat model of ulcerative colitis colon tissue nuclearfactor-kB (P50, P65) and protein expression level, which has great significancefor the of ulcerative colitis treatment.2. Lower normal colon tissue nuclear factor-kB (P50, P65) protein expression,and the untreated group, indicating that the nuclear factor-kB (P50, P65) andthe incidence of ulcerative and colitis was a positively correlated to guidedclinical practice.