| Objective:detection of patients with psoriasis vulgaris peripheral blood TGF-number of Th17and Treg and related factors in beta, IL-17, IL-22, IL-23levels, to analyze its correlation with different syndrome types and different disease, further clarify the pathogenesis of psoriasis, so as to achieve rich micro dialectical method with psoriasis vulgaris, provides the theory basis for innovation target therapy with psoriasis vulgaris.Methods:Selection of traditional Chinese medicine three syndrome types:intrinsic blood heat syndrome, blood deficiency wind dryness syndrome, stasis skin (PV) in patients with psoriasis vulgaris of the20cases,20cases in healthy volunteers as control group, respectively to extract each peripheral venous blood, two-color flow cytometry detection method of peripheral blood CD4Th17cells-FITC/IL-17-PE ratio, proportion of CD4-FITC/CD25-P Treg cells, as well as with double antibody sandwich ELISA method to detect serum TGF-beta, IL-17, IL-22, IL-23expression level.Results:Each card type and duration of Th17/Treg imbalances. Each card type comparison Th17proportion are higher than normal control group, the proportion of Treg are lower than the normal control group, the percentage of Th17cells in the blood hot intrinsic group (36.98±3.62), blood deficiency wind dry permit (17.45±2.49), skin stasis syndrome (12.65±3.88), the card type comparison were statistically significant (P<0.05, the proportion of Treg blood heat intrinsic development period (12.66±5.64), blood deficiency wind dry permit (4.32±2.55), skin stasis syndrome (7.88±2.71), compared with normal group(19.62±1.68) ratio is decreased obviously. The course of group comparison Th17cells percentage progress (28.66±3.24), stable group (19.22±4.15) and regression (12.48±2.35), the card type comparison were statistically significant (P<0.05, the proportion of Treg progress period (5.43±2.82), stable group (12.41±3.13) and regression (14.16±4.08), compared with normal group (19.62±1.68) ratio significantly decreased; Immune cytokine levels each syndrome group were higher than that of normal control group, the blood hot implication in the group of20patients with psoriasis were immune cytokine IL17(66.73±15.36), the highest level dominant; Blood deficiency wind dry IL22in the group of20patients (46.14±4.01) for level the highest levels, stasis group skin IL23(39.62±9.14) for level the highest levels, dominance, differences between groups were statistically significant (P<0.05). Cytokine levels compared to the course of the disease group were higher than that of normal control group, the progress in the phase of the group of20patients with psoriasis were immune cytokine IL17(53.13±5.22), the highest level dominant; Stability of group of20cases IL22(39.12±6.14) for level the highest levels, regression period of IL23(26.14±3.15) for level the highest levels, dominance, differences between groups were statistically significant (P<0.05). Card type immune factors have positive correlation with course of immune factors level. Th17cells was positively correlated with the patients’PASI score (r=0.49, P<0.49) with the course of the correlation (r=0.26, P<0.05).Conclusion:Th17cells, Treg cells and related immune cytokines play an important role in the pathogenesis of with psoriasis vulgaris, and can be used for immunological indexes to judge the severity of disease, further research helps innovation target therapy with psoriasis vulgaris.
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