Effect Of Ruanjian Xiaoging Granule On T Lymphocyte Subsets In EAT Rats

Purpose：To verify the effects of Ruan Jian Xiao Ying decoction in treatment of CLT. Bycontrasting the positive Tripterygium glycosides Tablet, we observe the effects of Ruan JianXiao Ying decoction on T-lymphocyte subsets of EAT rat’s PB and the weight of Thyroid.Material and method：Weigh sixty rats, and randomly divided into four groups byweight.(namely normal group, model group, Tripterygll Totorum treatment group, Ruan JianXiao Ying decoction treatment group). Each rat is marked by picric acid. After a week’s feed,the other three groups’ rats will be injected TG and CFA mixed solvent twice into abdominalsubcutaneous part. From the second week to the sixth week, TG and CFA mixed solventagain together with0.064%sodium iodide. Treat the rats for six weeks with TripterygllTotorum and Ruan Jian Xiao Ying decoction. Based on the testing:(1) Adopt ELISA to testTgAb and TpoAb.(2) Adopt ELISA to test T-lymphocyte subsets CD4+and CD8+in PB andcount CD4+/CD8+.(3) Weigh the rat’s Thyroid and calculates the index of Thyroid andweight. Method: Use spss17.0to analyze the results.Results:1. Variation of TgAb and TpoAb: TgAb and TpoAb have increased significantly inboth normal group and EAT group, and is of significance (P<0.01). This means rats’ model ofCLT is a success. There is no significant differences between Tripterygll Totorum treatmentgroup and Ruan Jian Xiao Ying decoction treatment group（P>0.05）. There are significantdifferences between the treatment groups and the model group（P<0.01）. This means bothgroups can decrease the double antibody in serum, which has effect on EAT rats.2. Variation of CD4+、CD8+and CD4+/CD8+: The model group has great difference from thenormal group (P<0.01). CD8+in the model group is lower than the normal group’s (P<0.01);CD4+/CD8+is higher than normal group’s and has significant difference (P<0.01); CD4+andCD8+in Tripterygll Totorum treatment group and Ruan Jian Xiao Ying decoction treatmentgroup has no great difference from the normal group’s (P>0.05); both treatment groups’ CD8+are obviously higher than the model group (P<0.01); CD4+/CD8+is obviously lower than themodel group (P<0.01); Comparing Ruan Jian Xiao Ying decoction treatment group with Tripterygll Totorum treatment group, CD4+、CD8+、CD4+/CD8+have no obvious distinction(P>0.05).Conclusion:1. TgAb and TpoAb in Rats of model group have significantly increased, which indicates thesuccessful creation of Eat rat; Ruan Jian Xiao Ying decoction treatment group and TripterygllTotorum treatment group can help lower the serum antibody in EAT rat, which indicatesRuan Jian Xiao Ying decoction can prevent CLT;2.Restore T Lymphocyte subpopulation CD4+/CD8+to a normal range and balance theimmunity response.