| Purpose:Purpose of this study was to from somatic level observation of skin photoaging process in human dermal micro vascular endothelial cells(hdmec)damage and prescription of traditional Chinese medicine Taohong Siwu Decoction intervention effect. Simulation study of solar UV radiation, UVA irradiation hdmec induced cell injury after, use Taohong Siwu Decoction intervening factors observed effect of Taohong Siwu Decoction containing serum on hdmec secretion of TGF beta 1 and transforming growth factor beta 1 receptor expression, to from the cellular and molecular level to clarify Taohong Siwu Decoction recume dermal microvascular endothelial cells injury mechanism, Taohong Siwu Decoction anti skin photoaging research provides the experimental basis.Material and method :HDMEC will be divided into five groups, namely control group, model control group, blank serum group, serum group, retinoic acid group.Except for the blank control group, the other groups of cell lines were irradiated with UVA, causing the damage of cells, the dose of 36J/cm2. The 15 SD rats, were randomly divided into 3 groups, respectively, blank serum, serum group, retinoic acid group. Were given saline, Siwu Decoction, retinoic acid after intragastric administration, extraction of rat serum. Model control group was irradiated with fresh culture medium and culture; blank serum group after irradiation with blank serum intervention; drug containing serum group after irradiation with Taohong Siwu Decoction containing serum intervention; retinoic acid group irradiation after retinoic acid containing serum intervention. After 24 h, the culture supernatant and cells were collected for the detection of related indicators.The contents of TGF- beta 1 in the culture supernatant of the cells were detected by ELISA, and the expression levels of R and TGF- II m RNA and R were detected by Western-blot and RT-PCR.Results:1 groups of cell culture supernatant of TGF- beta 1 content: compared with the blank control group, each group of cells in the supernatant of TGF- beta1 content decreased significantly(p<0.01), the difference was statistically significant; compared with the model group, serum andretinoic acid in cell culture medium of TGF- beta 1 was significantly increased(p<0.01), difference there is significant; compared with the blank serum group, serum and retinoic acid in cell culture medium of TGF- beta 1 was significantly increased(p<0.01), the difference was statistically significant.The level of TGF- beta R I m RNA expression in 2 cells in each group:compared with the blank control group, the expression of TGF- beta RIm RNA levels of each group cells showed low expression(p<0.01), withsignificant difference; drug serum group and retinoic acid treated cells with expression of TGF- beta R I m RNA levels higher than those in the model group(p<0.01), with statistically significant differences; drug serum group and retinoic acid treated cells the expression of TGF- beta RIm RNA level is higher than the blank serum group(p<0.01), withsignificant difference.The level of TGF- beta R I expression of 3 cells in each group: compared with the blank control group, a low level expression of TGF- beta RIexpression cell model group and blank serum group(p<0.01), statistically significant difference; level showed low expression of TGF- beta RI expression containing serum group and the retinoic acid group(p<0.05),with statistically significant differences; drug serum group and retinoic acid treated cells with expression of TGF- beta R I levels higher than those in the model group(p<0.01), with significant difference; level higher than the blank serum group TGF- beta RI expression containing serumgroup and retinoic acid treated cells(p<0.01), with significant difference.The level of TGF- beta R II m RNA expression in 4 cells in each group:compared with the blank control group, the expression of TGF- beta R IIm RNA levels of each group cells showed low expression(p<0.01), withsignificant difference; drug serum group and retinoic acid treated cellsthe expression of TGF- beta R II m RNA levels higher than those in the model group(p<0.01), statistically significant differences; drug serum group and retinoic acid treated cells with expression of TGF- beta R II m RNA levels higher than the blank serum group(p<0.01), with significant difference.The level of expression of TGF- beta R II 5 cells in each group: compared with the blank control group, the expression of TGF- beta R II level of each group cells showed low expression(p<0.01), with significant difference; drug serum group and retinoic acid treated cells withexpression of TGF- beta R II levels were higher than those in the model group(p<0.01), with statistical significance difference; drug serum group and retinoic acid treated cells with expression of TGF- beta R II levelshigher than the blank serum group(p<0.01), with significant difference.Conclusion:1. The UV radiation can make hdmec secretion of TGF- 1 content reduced, and inhibit the expression of TGF beta R I and II m RNA and protein, reduced TGF beta/smad pathway activity and thus influence to reduce the collagen synthesis, in order to determine the vascular endothelial cell injury and light ageing exists between the contact.2 Taohong Siwu Decoction and retinoic acid can promote the secretion of HDMEC TGF- injury and the increased expression of TGF- beta 1, beta R on m RNA and protein.The activity of TGF- /smad pathway was enhanced and the collagen synthesis of fibroblasts increased.3 the content of TGF- beta 1 decreased after injury of vascular endothelial cells,it may affect the collagen synthesis of fibroblasts and thus lead to skin light aging damage.
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