Effect Of Phytate On The Growth Of Colon Cancer HT-29 Cells By PI3K / Akt Signaling Pathway

Objective:this study was conducted to investigate whether the IP6 regulate the apoptosis of human colorectal cancer cell(HT-29)by inhibiting the PI3K/Akt signaling pathway.Method: human colorectal cancer cell(HT-29) was used for the study. The groups of each HT-29 cells were treated with IP6 0, 50, 100, 200, 400μg/ml,respectively. Then MTT colrimetric method was used to observe the proliferation of HT-29 in vitro and flow cytomtry(FCM) was used to analysis the apoptosis of the HT-29 Cell. The expression of related m RNA was determined by real-time quantitative RT-PCR,and related protein levels were analyzed by Western blot analysis.Result : HT-29 cells underwent inhibition of proliferation after exposure to IP6(100-400μg/ml) for 12 and 48 h, and this inhibition relied on time and dosage conspicuously. IP6 induce apoptosis in HT-29 cells in a dose-dependent manner. The m RNA and protein expression of PI3 K and Akt decreased in the groups treated with IP6(P<0.05), and IP6 inhibited the phosphorylation of Akt(p Akt) and increased expression of its downstream effector caspase-9(P<0.05), down-regulating the expression of m TOR and NF-κB(P<0.05).Conclusion:Our results suggested that PI3K/Akt pathway plays an important roles in IP6 induced apoptosis of human colorectal cancer cell HT-29.A dose of IP6 could inhibit the growth of HT-29 cells. The mechanism might bedown-regulating the expression of PI3 K,Akt,p Akt, m TOR and NF-κB.whereas increased the expression of its downstream effector, caspase-9.