| Background: Cerebral infarction,a type of ischemic stroke disease resulting from a blockage in the blood vessels supplying blood to the brain, is a threat to human health. Thrombolytic therapy and brain protection methods have been used to treat cerebral infarction clinically. But with the development of Brain Science and nerve regeneration, cell therapy and cell replacement therapy become a hotspot in recent years. This study aimed to explore the effect of placenta-derived mesenchymal stem cells on the neurological function of cerebral ischemia-reperfusion rats and the expression of caspase-3 in nerve cells.Methods: The rat cerebral infarction models were established. The treatment group was injected 2×106 Umbilical Cord Blood Mesenchymal Stem Cells;Sham-operated group and the control were injected into the same amount of cell culture media. Evaluation of neurological function was performed before and after the injection: 1day, 3days and 7days. The mRNA and protein expression level of caspase-3 was detected using RT-PCR and immunohistochemical method, respectively. Apoptosis analysis was assessed using a TUNEL kit.Results:Labeled Umbilical Cord Blood Mesenchymal Stem Cells survived well after being injected into rats; The neurological function of treatment rats improved compared with the control(F=29.996,t=2.236,n=6,p<0.05). RT-PCR and immunohistochemistry results indicated that the expression level of caspase-3 decreased in treatment rats(F=116.502~466.271, t=7.208~23.168, P<0.01), while there was no significant difference between the sham-operated group and the control(P>0.05). Apoptosis results showed that TUNEL-positive cells were significantly lower in the treatment group than the control group.Conclusions: This study indicated that placenta-derived mesenchymal stem cells contribute to the recovery of neurological function of cerebral ischemia-reperfusion rats and the expression of caspase-3 decreased in nerve cells. |
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