Effects Of Shidalazhiwan On The Expression Of Caspase-3 And Caspase-9 In Cerebral Ischemia Reperfusion Injury In Rats

Objective To study the effects of shidalazhiwan on the expression of cell morphology and Caspase-3 and Caspase-9 protein in Cerebral Ischemia Reperfusion Injury in Rats. In order to investigate whether the shidalazhiwan can go through the signaling pathway of PI3K/AKT to see the neuroprotective effect and its possible mechanism of cerebral ischemia reperfusion lesion,the author will use the method of observing butylphthalide injection pretreatment.Methods 169 male SD rats were divided into sham operation group and model group, shi da la zhi wan group,ginaton group,shi da la zhi wan+ginaton group at random, the model of middle cerebral artery occlusion(MCAO)were established by using the intraluminal suture occlusion method after four groups.After cerebral ischemia 2h to reperfusion in,according to the time points after 12h、24h and 72 h subgroups,a total of 13 groups,each group of 13.Observed the ultrastructure changes of the neurons in rat brain under transmission electron microscope, the expression of Caspase-3 and Caspase-9 protein were detected by immunohistochemical method and Western blot.Results1.Electronic microscope results showed:(1)compared with the false surgical groups,model groups the damage degree of cell、synapse and capillary were increased at any time point.(2)compared with model groups,ginaton groups the damage degree of cell、synapse and capillary were gradually restored at any time point.(3)compared with model groups, shi da la zhi wan groups the damage degree of cell、synapse and capillary were gradually restored at any time point, especially in the 72 h best to recover.(4)compared with ginaton groups, shi da la zhi wan groups+ginaton groups the damage degree of cell、synapse and capillary were restored the best at any time point,especially in the 72 h best to recover.2.Immunohistochemical staining revealed that:(1)compared with the false surgical groups, model groups protein expression of Caspase-3 and Caspase-9 significantly higher at any time point(P<0.01).(2)compared with model groups, ginaton groups protein expression of Caspase-3 and Caspase-9 significantly lower at any time point(P<0.05).(3)compared with model groups, shi da la zhi wan groups protein expression of Caspase-3 and Caspase-9significantly lower at any time point(P<0.05).(4)compared with ginaton groups, shi da la zhi wan groups+ginaton groups protein expression of Caspase-3 and Caspase-9 significantly lower at any time point(P<0.01).3.Western blot results show that:(1)compared with the false surgical groups, model groups protein expression of Caspase-3 and Caspase-9higher significant(P<0.01).(2)compared with model groups, ginaton groups protein expression of Caspase-3 and Caspase-9 slight decrease at any time point(P<0.05), while shi da la zhi wan groups protein expression of Caspase-3and Caspase-9 slight decrease at any time point(P<0.05),and one of them protein expression of Caspase-9 of the difference between group shi da la zhi wan 12 h and group false surgical hadn,t obviously statistical(P>0.05).(3) compared with ginaton groups, shi da la zhi wan groups + ginaton groups protein expression of Caspase-3 and Caspase-9 significantly lower at any time point(P<0.05).Conclusions1. To protect of shidalazhiwan on the cell morphology in Cerebral Ischemia Reperfusion Injury in Rats.2. Shidalazhiwan can suppression expression of Caspase-3 and Caspase-9,have control cell apoptosis of the Cerebral ischemia reperfusion.3.The Cerebral ischemia-reperfusion caused brain cells significant damage, shidalazhiwan could pretect brain function and inhibit brain cell apoptosis by activating PI3K/AKT signaling pathway.