Study On The Pharmacy

Herbal garden burnet has the effect that it can increase the number of white blood cell, widely used in clinical sanguisorba tumor patients treated by radiation and chemotherapy leukopenia caused by disease, has a definite curative effect. Study showed that saponins class composition is the main active site sanguisorba litres of white effect. But so far there is no garden burnet saponins effective parts of preparations. Therefore, this study intends to extract from Sanguisorbae, separation and purification of therapeutic efficacy of leukopenia effective part of Burnet saponin, and prepare a solid dispersion tablets, mainly includes the following six parts.1. Quality standards of SanguisorbaeBased on sanguisorba under regulation in China Pharmacopeia 2010. this study established the method of sanguisorba medicinal ingredients like saponins in thin layer identification and index component sanguisorba saponins-I method for the determination of the content.2. Preparation process for sanguisorba total saponins effective parts(1) Optimization of extraction for sanguisorba total saponins. By orthogonal test, with the yield of solids and solids in the transmission and the product of the content of total saponins as examining index, the effect factors of sanguisorba extraction technology was investigated. Determine sanguisorba total saponins extraction process is:Add the equivalent of medicinal materials quality seven times the amount of 85% ethanol reflux extraction 3 times, each time 1 hour.(2) Purification process for sanguisorba total saponins. The optimization method of macroporous adsorption resin purification sanguisorba saponins technological conditions and parameters. In sanguisorba the content of total saponins as examining index, the optimum resin purification:D101 macroporous resin, the sample liquid concentration is 0.5 g/ml, PH5-6, the adsorption rate of I bv/h, the sample is 2 times the amount of resin bed volume. Turn 5 by water and 40% ethanol elution in removing impurity, preoccupy seven bv70 the flow rate of 2 bv/h% ethanol elution, collect 70% ethanol elution liquid.3. Quality standards of sanguisorba total saponinsThe quality standards of sanguisorba total saponins was established, which mainly includes sanguisorba total saponin powder properties, check (moisture, heavy metals and arsenic salts and macroporous resin residue), thin layer identification and major component sanguisorba saponins -i content determination.4. Solid dispersion technology researchSolid dispersions prepared by melting method, with sanguisorba saponins -I dissolution as an evaluation index, optimum carrier material for PEG6000, best for 1:3 proportion of drug carrier.5. Formulation and preparation of dispersion tablets of BurnetTo adopt wet granule preparation solid dispersion tablets, taking tablet disintegration time limit and the dissolution of sanguisorba saponins-I as the main index, and the optimum prescription was obtained:microcrystalline cellulose as diluent,3% high inflating carboxymethyl starch sodium as disintegrating agent, and 1% magnesium stearate as lubricant.6. Quality standards research of dispersion tablets of BurnetThe quality standards of dispersion tablets of Burnet was established, which mainly includes character identification, identification (TLC), inspection(moisture, difference of weight, disintegration time, microbial limit) and established the content determination method for total saponins and Sanguisorba saponins-I of index constituents of dispersion tablets burnet.