Study On Chemical Composition And Anti - Inflammatory Activity Of Wild

Capparis spinosa L. was recorded in the Uighur Medicine Standard (issued by Department of Health) in 1999 edition, which belongs to Capparidaceae. The leaves, fruits and root barks of C. spinosa are usually used to treat rheumatoid arthritis (RA), which with the functions of depriving the evil wetness, detumescence and analgesia. As a Uighur medicinal herb, C. spinosa still has not got much attention, so its chemical compositions and pharmacological activities were seldom investigated in China. According to the isolation which was guided by activity, the present study was focused on the therapeutical basis of RA, and the results were made as follow:(1) Under the guidance of anti-inflammatory and analgesic experiments (xylene-induced mouse ear swelling and carrageenan-induced paw swelling in rats, acetic acid induced mouse writhing and hot plate test), the EtOAc and n-BuOH layers extracted from 75%ethanol extract of C. spinosa showed significant anti-inflammatory and analgesic action. The PE extract also showed anti-inflammatory effection.(2) 23 compounds were obtained from the bioactive parts by the methods of the silica gel, Sephadex LH-20, ODS and HW-40 column chromatography, and 17 of them were identified as 9,12-octade cadienoic acid (Z, Z) (1),9-octadecenoic acid (E) (2),9-octadecenoic acid (Z) (3), n-hexadecanoic acid (4),β-sitosterol (5),1,2-benzendicarboxylic acid, bis (2-ethylhexyl) ester (6), butanedioic acid (8),6-methyl-3-pyridinol (9),2-methylene-4-pyridinol (10), daucosterol (11), p-hydroxybenzoic acid (13), hexadecanoic acid,2-hydroxy-1-(hydroxymethyl) ethyl ester (14),5-(hydroxymethyl)furfural (15), bis (5-formy-lfurfuryl) ether (16), a-D-fructofuranosides methyl (18), uracil (19), stachydrine (23). Compounds 6,9,10,14,15,16,18 were first obtained from Capparis genus.(3) Based on the study of chemical constituents, the anti-inflammatory and analgesic activities of total alkaloids and stachydrine were investigated in this paper, and the results showed the notable activities. Therefore, total alkaloids and stachydrine were considered as acitive constituents of C. spinosa for curing RA. Stachydrine can also reduce the production of NO. The anti-inflammatory and analgesic activities of stachydrine may be related with above mechanism.