Determination Of Carbofuran Residues In Animal Tissues By Lipid Chromatography/Mass Spectrometry

N-methylcarbamat (NMCs) is an important class of pesticides which is widely used in gardens and agriculturally for the protection of a large variety of cropes. Carbamate drugs residue was short, efficient, and selectivity. Furadan (Carbofuran) is one of an efficient drug, which was broad-spectrum pesticides within the suction. Carbofuran is the current of domestic production and application of the main varieties of carbamate pesticides. Since this drug have been effective, inexpensive, easy to get, etc. It has been widely used to control grain, cotton, oil, sugar, tobacco, tea, fruits and other crops, ornamental plants and a variety of pests, mites and nematodes, However, Carbofuran is highly toxic pesticides, which is also animal pesticides, easily through the food chain, water and even air pollution to pollut livestock, furtherly, residues in animal tissues, human and livestock. Aquatic animals of high toxicity. Carbofuran caused by drug toxicity and residue potential food safety hazards can not be ignored. To solve the above problems and the community, the market's needs, this study of carbofuran residues in pig edible tissues need an efficient detection method. So the liquid chromatography-mass spectrometry detection methods was used, So the study of Carbofuran in animal is very important to the food safety. These results will provide right using method for Carbofuran in animal and science gist for the safety evaluation.A high performance liquid chromatography/electrospray ionization tandem mass spectrometric method (HPLC-MS) has been developed for the determination of Carbofuran pesticide residues in tissue of porcine.Tissue samples of porcine were subject to acetonitrile followed by liquid-liquid extraction and Oasis HLB solid-phase extraction clean-up. Animal tissue samples were extracted by acetonitrile extraction repeated twice, combined two of lotion, after bath rotary evaporation to dryness (enrichment steps to control the temperature does not exceed 40℃), washed with acetonitrile and evaporation flask 4ml twice, combined washing twice solution,5ml of n-hexane is added to the lipid, vortex mixing (1min), discard hexane layer and repeat the operation twice to fat,40℃dried by nitrogen, add 5ml of water and mix..Aqueous solution and solid phase extraction cartridge HLB cleaned, washed with 3ml acetonitrile, collecting eluate 40℃dried by nitrogen. After elution with 0.5ml of mobile phase (methanol:10mmol/L ammonium acetate=65:35) solution concentrated residue, over 0.22μm membrane and liquid chromatography-mass spectrometry detection.The results showed that in pig muscle Carbofuran limit of quantification was 0.5ng/g, in the pig liver and kidney in the quantitative limit is 1ng/g and 1ng/g.; When the muscle Carbofuran concentration was 0.5ng/g, 1ng/g,2ng/g, the recoveries for the 74.92%-79.60%, day coefficients of variation range of 5.78%-8.95%; When the liver Carbofuran concentration was lng/g,2ng/g,5ng/g, the recoveries were 73.77%-78.48%, day coefficients of variation range of 6.73%-9.32%. When the kidney Carbofuran concentration was lng/g,2ng/g,5ng/g, the recoveries for the 74.06%-76.15%, day coefficients of variation range of 6.28%to 11.19%. Carbofuran standard solution in 0.1-100.0ng/mL, its regression equation y=814.26x+1236.5, the correlation coefficient r =0.9995. Add the pork muscle concentration of carbofuran in 0.5ng/g-8ng/g, the regression equation of working curve y= 9712x+822.3, r=0.9993; pig liver concentration range of added carbofuran lng/g-16ng/g when Working curve regression equation y=5123x+413.71, r=0.9994; pig kidney concentration range of added carbofuran lng/g-16ng/g, the working curve regression equation y= 5858x-2392.1, r=0.9992. Thus, accuracy and precision of quantitative analysis are in line with the requirements, and the selected concentration range, linearity better.This simple sample preparation method, exhibits that high sensitivity. These results are declared national standard, widely used in tissues of porcine. At the same time, Carbofuran residues. can be used as the detection of other animals or tissue; carbamate drug residue reference method also was used to ensure the food safety.