The Establishment Of Micropropagation Technical System Of Cercis Canadensis 'Forest Pansy'

Cercis Canadensis'Forest Pansy'stem with axillary bud was used as explant. Tissue culture of Cercis Canadensis'Forest Pansy'was studied systematically in this paper in order to set up techniques for commercial production. This study could provide technical support for molecular breeding and related studies in this field.In this study, the nodal position, collecting time, degerming method, browning control method, types of plant growth regulators, the optimal initiation medium, proliferation medium, rooting medium, acclimatization disposal and transplant in Cercis Canadensis'Forest Pansy'tissue culture were studied systematically. The results were as follows:1. The optimal collecting time of explants was from the last ten days of April to June. The optimal nodal position was the middle stem ( the third and forth nodes ).2. The effective sterilization procedure of explants was as follows: the axillary buds of Cercis Canadensis'Forest Pansy'→washed up by tap water→dipped in the detergent water for about 10min and used a soft brush to clean the surface dirts→washed up for about 30 min by tap water→put on the clean bench→dipped in 70% alcohol for 30s→dipped in the 0.1%HgCl2 for 5min→washed 5 times by asepsis water→both ends of explants were cut 1.5cm individually. The survival rate of explants was 93.3%.3. The optimal initiation medium in tissue culture of Cercis Canadensis'Forest Pansy'was MS+6-BA 1.0mg/L+NAA 0.1mg/L+AC 0.6mg/L+Sugar 20g/L+agar8g/L。4. The optimal initiation medium was DKW+TDZ 0.03mg/L+NAA 0.1mg/L+PVP 1.0mg/L+Sugar 20g/L+Agar 8g/L. The proliferation coefficient was 4.27.5. The optimal medium for strong seedlings was MS+6-BA 1.0mg/L + NAA 0.1mg/L+ AC 1.0g/L+ Sugar 20g/L+ Agar 8g/L.6. The main factors which influenced rooting were medium, NAA and IBA. At the initial period of rooting, the optimal medium for rooting was 1/2MS+IBA 0.5mg/L+AC 1.0mg/L+ Sugar 20g/L+Agar 8g/L. At the later period of rooting, in order to increase the number and the length of roots, the medium with 1/2 MS + IBA 25mg/L + AC 2.0mg/L + Sugar 20g/L + Agar 8g/L was a better choice.