Study On Tissue Culture And Rapid Propagation Of Actinidia Deliciosa

Kiwifruit is popular because of its high vitamin C content and sweet flesh.There are many disadvantages in traditional seedling raising methods.The tissue culture method of modern biological technology can not only overcome the disadvantages of traditional seedling raising,but also help to maintain the good characters of parents,establish aseptic seedling system,quickly and efficiently breed stable seedlings,realize rapid promotion and application,and help promote regional economic development.Xuxiang kiwifruit,as a good variety with great potential confirmed by the market,has been regarded as an important variety of kiwifruit industry in Xixia.However,the traditional seedling raising method has a long cycle,and the seedlings are seriously susceptible to diseases and poor growth,which has a great impact on production.In this experiment,the stem section of axillary buds of kiwifruit was used as the explant of tissue culture,to establish the tissue culture and rapid propagation technology system of kiwi fruit.At the same time,many factors influencing the establishment of rapid propagation system were explored and optimized,so as to realize the precise control of tissue culture process as far as possible,which laid a foundation for factory breeding seedlings.The main results are as follows.1.The best disinfection method for stem segment of kiwifruit 75%alcohol for 30 s+0.1%Hg Cl2 for 8 minutes,the survival rate was as high as 60.00%,the pollution rate and browning rate were 20.00%,both of them were the lowest value.2.The best medium for inducing axillary buds was MS+3 mg/L 6-BA+0.2 mg/L NAA,and the highest germination rate of axillary buds was 90.00%.3.The best medium for proliferation culture was MS+5 mg/L 6-BA+0.5 mg/L NAA,and the average proliferation coefficient was 3.23.4.The best medium for direct induction of adventitious buds using leaf disc was MS+3 mg/L 6-BA+0.3 mg/l NAA,the germination rate was as high as 90.00%and browning rate was only 10.00%;The best medium for the induction of adventitious buds by petiole was MS+3 mg/L 6-BA+0.3 mg/L NAA,the germination rate was 86.67%,and the browning rate was only 13.33%.5.The best medium for strong seedling culture was MS+1.5 mg/L GA₃,the height of buts reached the maximum value of 4.50 cm,stem diameter could reach 2.45 mm,the maximum proliferation coefficient reaches 5.37,and the growth trend of bud was better.6.The best medium for rooting was 1/2 MS+0.7 mg/L IBA,and the rooting rate was100%.7.The best time to open the cover and refine seedlings before transplanting was 5 days;when the ratio of river sand,humus soil and vermiculite was 1:1:1,the survival rate of transplanted seedlings was the highest,up to 93.33%.