Studies On The Cytological Damage Effeets By Low Energy Lon Implantation In Rice

The damage of the root tip cell chromosomes, including the rate of distortion, the type of aberration and the mitotic index etc. of the Ml and M2 generations coused by low energy ion implantation was studied in this paper, using diploid and tetraploid rice as materials. In addition, the distribution pattern of microfilaments network of the rice root tip cells during differentiation are preliminary researched. The results of this study was as follows.1. Implantation of low energy N+ ion beam can cause different types of chromosomal aberrations of the M1 root tip cells in mitosis, such as chromosome bridge, chromosome small fragments, laggards, etc. Chromosome aberration rate in root-tip cells is not proportional to N+ ion treatment dosages. but showes some randomness. Low energy N+ ion beam Implantation on some rice varieties mitotic of root tip cells could promote the mitotic index of Ml generation root tip cells increased. Some injected dose can promote mitosis of M1 generation rice strains root-tip cells, and some doses inhibited mitosis of root tip cells.2. Low energy N+ion beam implantation on M2 generation rice varieties mitotic of root tip cells also could promote, and lead to M2 generation root tip cells of the chromosome aberration, the main types of distortion, chromosome bridge, chromosome small fragments, laggards, etc. M2 with Ml generation root tip cells of chromosome aberration of a similar type. M2 generation of root tip cell chromosome aberration rate lower than the M1.3. Seeds of rice treated by low energy N+ion were planted at rice experimental field of Institute of Rice Science, Xinxiang Agricutural Technology Research Academy. The meiosis of pollen mother cells in M1 generation were observed. The result showed that the chromosomal aberrations were similar to the type of mitotic aberration, appearing chromosome bridge, small chromosomal fragments, lagging chromosomes. Besides that, the free chromosomes, chromosome separation and other special phenomenon ahead of schedule were found.4. The distribution pattern of microfilaments network of the rice root tip cells during differentiation are preliminary researched. It was found that the actin distribution pattern in rice root tip cells during differentiation from meristematic zone to the mature region were significant different. There were two states of actin distribution in the meristem region. In interphase cells, There were no obvious microfilament network, the cell was inspired by the uniform, bright green fluorescence, and the nucleus is clearly visible. In mitotic phase cells, nucleus were surrounded by a large number of bright fluorescence, Showed a "cage"-like distribution. There were a large number of fluorescent nuclei peripheral radial distribution of the four presented in elongation region cells. The nucleus were surrounded by bright fluorescence, while almost no fluorescence in cytoplasm in mature zone cells.