Study On Ultra-dry Storage Of Several Tree Seeds In Three-north Area Of China

Seed is the principal part of plant germplasm resources, because the protection of seed has a perfect structure and adapt to the environment inherent in the delicate mechanism, setting up to collect seeds for the main plant germplasm preservation of germplasm resources is the common and easy way for most species kept, because seeds have a perfect structure and internal. adaptive mechanism. The technique of Ultra-dry seed can maintain the genetic integrity, improve the storage tolerance of seeds and has a widely application prospect in the field of plant germplasm conservation. Larix olgensis Henry,L. gmelini Rupr.,L. principis-rupprechtii Mayr.,L. kaempferi Carr.,and Picea asperata Mast.are the important plantation tree species in Northeast and North of China, and Hippophae rhamnoides Linn.has important economic value and environmental value in Northwest of China. The various index of seeds which after ultra-drying (before and after aging) were investigated. Further more, the study can provide a theoretical basis of the several species seeds'germplasm preservation in three-north area.With the method of drying by silica gel, L. olgensis Henry seed MC were dried from 7.1% to 5.0%,3.9%,3.0% and 2.3%; L. gmelini Rupr. seed MC were dried from 5.7% to 4.1%, 3.2%, 2.6% and 1.9 %; L. principis-rupprechtii Mayr. seed MC were dried from 6.7% to 5.0%, 3.7%, 2.8% and 2.3%; L. kaempferi Carr. seed MC were dried from 8.4% to 4.8%, 3.8%, 2.8% and 2.2%; and P. asperata Mast. seed MC were dried from 8.2% to 6.4%, 5.2%, 4.1% and 2.9% and H. rhamnoides Linn. seed MC were dried from 8.4% to 6.8%, 4.4%, 3.4% and 2.4%. After ultra-drying (before and after aging), germination energy (GE), germination percent (GP), germination index (GI), vigor index (VI), relative conductivity (RC), dehydrogenase (DH) and isocitratelyase (ICL) activity orα-amylase activity(α-AA) were investigated. The results are as follows: 1 L. olgensis Henry, L. gmelini Rupr., L. principis-rupprechtii Mayr., L. kaempferi Carr., Picea asperata Mast. and Hippophae rhamnoides Linn. seeds were dried to 5% moisture content (MC) or less in by the silica gel method is feasible.2 The order of seed drying velocity is L. kaempferi Carr.> P. asperata Mast.> L. principis-rupprechtii Mayr.> L. gmelini Rupr. > L. olgensis Henry > H. rhamnoides Linn.3 Seeds with various moisture contents have different ability of deterioration-resisting. And seeds with low moisture contents have stronger ability of deterioration-resisting than seeds with high moisture contents have. For L. olgensis Henry seeds with 3.0% MC, L. gmelini Rupr. seeds with 2.6% MC, L. principis-rupprechtii Mayr. seeds with 2.8% MC, L. kaempferi Carr. seeds with 2.8% MC, P. asperata Mast. seeds with 4.1% MC, and H. rhamnoides Linn. seeds with 3.4% MC, after aging, their GP, vigor and physiological and biochemical indexes are higher than those of seeds with initial moisture contents, and their RC is lower than the latter's.The seeds showed stronger anti-aging ability than initial moisture contents.4 After moisture content drying under 5%, kinds of seeds keep high level of germination percentage and vigor, their ability of deterioration -resisting is stronger than that of seeds with initial moisture content, and their physiological characteristics are not changed compared to seeds with initial moisture content. Their optimum moisture contents are 3.0% MC for L. olgensis Henry, 2.6% MC for L. gmelini Rupr., 2.8% for L. principis-rupprechtii Mayr., 2.8% for L. kaempferi Carr., 4.1% MC for P. asperata Mast., and 3.4% MC for H. rhamnoides Linn..