Study On The Optimization Of Liquid Fermentation Process Of Shiraia Bambusicola And Extraction Of Hypocrellin

Zhuhuang, a famous Chinese tradional medicine, is the stroma of shiraia bambusicola P. Henn. It has the effects of cough relieving and sputum reducing, blood circulation and relax emmenagogue, acetanilide invigorate the circulation of blood, help to organize the recovery of function and invigorate health effectively. The development of Zhuhuang is very attractived continuously developing and studied deeply. However, because of harvesting time and areal distribution, the natural resources are limited, and it become less and less in quantity. So the artificial culture has great significance.This paper discussed the high yield of hypocrellin achieved by optimizing the liquid fermentation technological process. The results were showed as follows:1. There is a significance difference of strains between tissue isolation and spores isolation after liquid fermentation culture. The mycelial pellets of tissue isolation is large in shape but few in quantity, while the mycelial pellets of spores isolation is large in quantity but small in shape. But in the yield of hypocrellin, the extract of the 2 kinds of liquid fermentation culture is different. The former yield is 104.42mg / L, while the latter yield is 72.82mg / L.2. The molecular weight of compound I is 546 and compound II is 528 by MASS(ES+,ES-)Test, which is the same as the molecular weight of hypocrellin A and hypocrellin B respectively.3. The Optimal liquid fermentation condition to produce hypocrellin A and hypocrellin B is as follows: Potato: 160g / L, brachystachyum juice: 8g / L, sugar: 10g / L, Sodium nitrate: 2 g / L, pH :6.0, Temperature: 26℃, 1 / 5 liquid volume, 120r / min , constant temperature cultivating time : 96h. The yield of hypocrellin A is 156.56mg / L, hypocrellin B is 178.23mg / L, total yield of hypocrellin is 334.79mg / L under the conditions.