Cultivating The New Line Of Disease-resistant Tomato By The Technology Of Transgenic

Tomato(Lycopersicon esculentum.Mill) is an important economic crop, rich in vitamin C and trace elements, with high nutritional value and large-scale cultivation in China. It can be used as vegetables, can be eaten as fruit, can also be processed into tomato paste, tomato juice cans products, export earnings, with high economic value. Tomato yield and quality will directly affect the development of agriculture and food industry, therefore, improving the yield of tomato and improving the quality of tomato have great significance to our province and China's economic development. However, the growth process in the tomato, pest and disease problems are still more prominent. The fungal diseases currently affecting tomato are mainly tomato early blight, late blight, leaf mold, gray mold, fusarium wilt, umbilical rot diseases, and so on. These diseases often occur in tomato at all stages from seedling to adult stage, harm tomato root, stem, leaf, flower, fruit and other organs, which seriously affect the growth of tomato and destroy the quality of tomato. The national economy thus suffer a major loss. The current main method of controlling plant fungal diseases is spraying chemical pesticides, but this approach will ultimately lead to enhanced resistance to pathogens and pesticide, and residues also cause environmental pollution and do harm to human health.Chitinase has a confining role on fungi containing chitin on the cell wall. It has been found that many animals, plants and microbes can produce chitinase. With the deep study of chitinase, chitinase in plant disease resistance gene engineering has been widely carried out together. The purpose of this study is to use the new chitinase gene cloned from trichoderma andβ-glucanase gene cloned from tobacco, lead these two resistance genes into tomato by genetic engineering methods and cultivate new disease resistance germplasm or new line. The project will first explore the tomato regeneration system and related technologies and lay the theoretical and technical foundation for tomato cell engineering and gene engineering research. The results will help to resolve the serious tomato disease problems, help to improve tomato production, improve the quality of tomato and promote the sustainable development of agriculture and food industry. Transgenic tomato with a strong resistance to diseases, can significantly reduce the use of chemical pesticide, reduce the human, material and financial resources in tomato cultivation and reduce production costs. Since it can reduce the environmental pollution and harm to human health of chemical pesticides, the results will benefit the environmental protection. Therefore, the study will have a better theoretical and practical value.Through the deep study of several main cultivated varieties of tomato according to tomato cultivation in Shandong, we make use of transgenic technology, design experimental methods and technical line and complete the following work:1. Determine the 2 tomato varieties with strong leaf regeneration ability through tissue culturing for several main cultivated tomato varieties in Shandong;2. Lead exogenous resistance genes into tomato leaf by the method of agrobacterium-mediated and screen transformed callus;3. Acquire transgenic plants by leaf regeneration and conduct the transgenic plants PCR detection and Southern interbreeding detection;4. Conduct resistant test, screen the transgenic plants and acquire new lines of tomato with high resistance to disease.In the process of completing the above work, the paper mainly focuses on the following three innovative researches:1. Study the tissue culture and plant regeneration technique of the 11 main cultivated tomato varieties in Shandong and identify two species with strong regenerative capacity.2. Put the two antifungal genes into tomato at the same time and acquire the enhanced resistance in transgenic tomato lines.3. Summarize a set of experimental tomato regeneration and transformation technology and provide a basis for the further research of tomato cell engineering and genetic engineering.Cell engineering breeding is an important breeding method, which is difficult to replace by other biological technologies and should be continued to develop. We make a study of tissue culture and plant regeneration for 11 tomato varieties, or 11 different genotypes, explore the high-frequency regeneration methods system, find that significant differences exist between different species, and screen out 2 varieties of tomato of high regenerative capacity, which provides a good experimental material for further study of engineering and genetic engineering.