Study On Population Ecology And Assessment Of Germplasm Resources Of Taxus Chinensis Var. Mairei

Taxus chinensis var. mairei, being one of the state-protected one-grade rare and endangered plants, is one of the tertiary relic species and endemic to China. It is also distributed as one of the most wide plant in the taxus. Based on the field investigation, T. chinensis var. mairei was studied by using quantitative ecology methods, including numeric dynamics of population, spatial distribution pattern, innerspecific relationship etc, and assessment of its germplasm resources. The aim of this study was to reveal its endangered condition and reasons, promote some theory proof so as to protect this endangered species from damage, product plantation and utilize this resource sustainably. The main results of this research could be concluded as followings:1.The population dynamics of T. chinensis var. mairei population in the Mountain Area of Southern Anhui Province were analyzed by using the method"diameter structure replacement age structure", and the life table was formulated, survival was employed. The rusults showed that this population was in developing tendency with no declination. But there had a high peak of death rate of the population appeared in the age class 1, and only 16 % young could enter the age class 2. It suggested that there was a stronger environmental sieve in the process from seedling to juvenile stage which restricted individual growth and development so as to cause most of seedling death.2. The analysis and the discussion on the spatial patterns of different age-class individuals of T. chinensis var. mairei populations in the mountainous area of southern Anhui Province were made by O-ring statistics—one method of point pattern analysis, using Programita software. The result showed that the Xianyu population had the spatial distribution pattern of randomly distributing at scales 2 to 25 m, but of clumped at scales< 2 m, while the Xiaxiang population tended to be randomly distributed at all scales studied. The age-classⅠof Xianyu population was clumped at scales< 5m, while the older age-class individuals tended to be non-randomly distributed in some scales. It might be related to the feature of population regeneration in Xianyu population.3. The spatial association between different age-classes of T. chinensis var. mairei in that two population will turn to be negative if their age-class differences are increasingly enlarged. No significant correlation, however, was observed between closer age-classes. This spatial association of individuals in Taxus chinensis var. mairei population is beneficial to the effective use of resources, and there has been a process by which the spatial distribution pattern of the species population transforms from the clumped into the random due to the changes of individual growth.4. Using the chromatograms with zonal electrophoresis, the analysis was performed on the buffer solution was 35 mmol/L borax solution, the separation voltage was 18 kv, the detection wavelength was 228 nm. And then the fingerprint of T. chinensis var. mairei was built up with HPCE. The analysis indicated that the regional environment was the main influence factor for the quality of T. chinensis var. mairei.In addition, some suggestions and strategies were discussed for protecting the rare species based on the situation of T. chinensis var. mairei in the field.