Ananlysis Of Transcriptome Differences Between Tpa And Its Wild Type Based On The Microarray In Turnip

Recently, research on gynoecium development has been one of the highlights in plant developmental biology for last decade, and there are a lot of progress made in the genetic pathways underlying the initiation and development of the gynoecium to date. However, more efforts are needed to unravel the puzzles of gynoecium development. Our laboratory obtained a tpa (turnip pistil abortion) mutant which was employed to explore the genetic mechanism of gynoecium development in Brassica crops. Some interesting results have been obtained as follow:(1) After morphological investigation and microscope observation, we found the phenotpye of tpa is unique in the turnip. In this tpa mutant, sepal, petal and stamen are not affected. However, most of the gynoecium in the tpa plant began to shrink during the early development, finally the shape of pistil turn into filiform, moreover, lots of flowers even lack of pistil completely. Using the pollen of tpa plant cross with wild type plant, the tpa mutant character can be inherited successfully. The segregation ratios of the self cross for wild type plant were the following forms:0,1:4.3,1:1.3,1:11,1:15,1:28. And the segregation ratios of the offspring for tpa plant crossed with wild type plan were the following forms:0,1:1,1:1.3,1:1.4,1:1.5, 1:2.3. Our data suggests the tpa mutant is not controlled by single recessive gene,but most likely controlled by two recessive genes.(2) Global analysis the genes expression difference during the gynoecium development in turnip. Through the Jinxin (?) 29K microarray (The Arabidopsis thaliana Genome Oligo Set Version3.0) analysis, we found some potential gene related to gynoecium development. According to the data came from microarray analysis, there were 94 differential genes,63 genes were up-regulated and 31 genes were down-regulated in the floral buds of tpa plants. We choosed 26 genes for RT-PCR analysis, the results show there were a few difference of these genes expression between the floral buds of tpa and wild type plants. Fourteen genes expressed specifically in the floral buds, and the other 12 genes all expressed among the floral buds, stems and leaves with-no remarkable difference.(3) The Arabidopsis ATH1 genome array was employed to analyze the transcriptome differences between the tpa mutants and their wild type flowers. Data analysis results indicated there were differences in 152 genes between the wild type (W1) and the pistil aborted completely (M3), likewise,61 genes with transcriptional difference between the W1 and the pistil aborted partially (12), and 24 genes with transcriptional difference between the 12 and M3. All of the previous genes were classed in functional category as follow:cell wall biosynthesis and regulation, defence and stressing reaction, transcriptional regulation, protein metabolism and normal metabolism, respectively. Out of 41 genes,9 genes with significant temporal and spatial transcription difference:(At2g42840, At1g57750, At5g20630, At2g03090, At3g08030, At5g08000, At2g28790, At5g63310, At2g24270), have beeen obtained through the RT-PCR screening and confirmation.Research of genetic characteristics and genes which involve in the gynoecium development in tpa mutant are still in progress.