Technology Research Of Rapid Propagation Of Excellent Clones Of Eucommia Ulmoides Oliv

Eucommia ulmoides Oliv is an unique medical economic tree species of our country, plant of the second-class protection. Its'unique ingredient gutta-percha is one kind of hard rubber, which is corrosion-resisting, having good insulation energy, good plasticity, and high pressure resistance intensity. As a medicinal plant, it can reduce blood pressure, diuresis, improve dizziness and losing sleep and some other sicknesses.Eucommia ulmoides Oliv can produce in the natural and vegetative way. Mostly, it produces by the seed multiplication. But the seed-germination percentage is not high, and the component of medical effect and gutta-percha difference between individuals of direct seed descendant is remarkable, hinders the promotion of newly selected breeds which contains more medical components and gutta-percha.In order to accelerate the promotion of fine breeds of Eucommia ulmoides Oliv HuaZhong No.1, seeds, embryo of different phrases, dormant buds in winter and young shoots of spring were used as raw material, tissue culture of Eucommia ulmoides Oliv was conducted. Establish technique of tissue culture in direct and indirect organgenesis path using mature embryo as explant, and acquire embryogenic callus using immature embryo as explant. Focus on medium formula of each stage, orthogonal comparison and spss were used to the data processing. The results were as follows:1. Direct organgenesis:Acquire regeneration plant in direct organgenesis path using mature embryo as explants. The optimum initial culture medium for buds was: MS+BA(0.5mg/L-2.0mg/L), inductivity was 50%.The optimum medium for adventitious buds proliferation was:MS+BA2.0mg/L, proliferation index was 162.5%.2. Indirect organgenesis:Acquire regeneration plant in indirect organgenesis path using mature embryo as explants. The optimum initial culture medium for callus was: MS+BA(2.0mg/L)+NAA (0.1-2.5 mg/L), inductivity was 70%. The optimum medium for callus proliferation was:MS+BA (2.0mg/L)+NAA (0.5-2.5 mg/L), proliferation index was 180%. The optimum medium for adventitious buds induction was:MS+BA 2.0mg/L, inductivity was 80%.3. Embryogenic callus:Using immature embryo as explants. The optimum initial culture medium for embryogenic callus was:MS+BA 1.0mg/L+2,4-D 2.0mg/L. The optimum culture medium for maturing of embryogenic callus was:MS+ BA 0.5mg/L +NAA0.1mg/L.4. Rooting, transplant:The optimum culture medium for rooting was:1/2MS, rooting percentage was 80%.The matrix of transplant was peat and perlite of same volume, survival rate was 67.7%.