Studies On Ginger Oil Extraction And Chemical Components And Its Antibacterial Activity From Zingiber Officinale Roscoe

Ginger was used for identifing the optimum extraction conditions of ginger oil by orthogonal test in this experiment. The ginger oil extraction with ethanol and supercritical CO2 fluid were conducted, and the antioxidative activities and bacteriostatic activity of the extractions were also studied in this research. The results are showed as follows.1. Extraction with ethanol. The highest extraction ratio of gingerol reached 1.61% under the conditions of 50℃,60-meshes screen,1:10 solid-to-liquid ratio, 2-hour-extraction time using 80% ethanol as extraction medium.2. Extraction with supercritical CO2 fluid. The extraction ratio ginger oil reached the highest of 5.38% under the condition of 22Mpa pressure,50℃,50m3·h-1 CO2 flow rate,15min with supercritical CO2 fluid extraction. The components of ginger oil identified by GC-MS method were zingiberene, curcumen zingiberon, P-sesquiphellandrene and zingiberol, etc.3. Antioxidative activity of ginger oil. The antioxidative activity of ginger oil increased with concentration within the range of 0.05%-0.5%. The antioxidative activity of ginger oil was higher than 0.2% ascorbic acid, and was equivalent to 0.2% Vitamin E. The antioxidative activity of 0.5% ginger oil was slightly higher than 0.02% BHT and 0.02% BHA. Additional test results showed that antioxidative activity of ginger oil to vegetable oil was higher than that to animal oil.4. Bacteriostatic activity of ginger oil. Ginger oil could inhibit the growth of Escherichia coli, Staphyloccocus aureus, Salmonella S.enterica, Shigella dysenteriae and Bacillus thuringiensis, especially to Shigella dysenteriae and Bacillus thuringiensis. Minimal inhibitory concentrations (MIC) to Escherichia coli, Staphyloccocus aureus and Salmonella S.enterica was 0.1 g-mL'', and to Shigella dysenteriae and Bacillus thuringiensis was 0.05 g·mL-1, Further research showed the ginger oil still had certain inhibitory effect under 40℃～120℃or or after 10min～120min of ultraviolet radiation.. It was suggested the ginger oil has a certain thermal stability and photostability respectively.