The Expression Of Tubby-like Proteins In Rice Leaves And During The Interactions Between Rice And Xanthomonas Oryzae Pv. Oryzae

Background: Rice(Oryza sativa)is a very important crop, rice disease can reduce the yield of rice, it can even result in the lost of harvest. Rice bacterial blight is one of the most important rice diseases.The study to the mechanism of rice disease resistance gene has great significances, The study can benefit us of protecting rice from destroy, enhance rice yield and solve the problem of world people's famine.The universal existence of tubby-like proteins (TLPs) within animal and plant genomes implies that TLPs may play key roles in life processes.Aim: The generation of specific antibodies against rice Tubby-like proteins to investigate the expression profiling of rice Tubby-like proteins in different stages of rice leaves and accumnulate basic information for functional studies.Materials and methods: Epitopes were predicted for candidate gene-encoded proteins and syntheticed high peak value peptides. Polyclonal antibodies were generated by rabbit immunization. Western blotting analysis were carried out for rice material collected at different developmental stages, including seeding stage 1cm, 2cm, 5cm, 10cm, 15cm, tilling stage, booting stage, flowering stage, filling stage. Comparison analysis with transcriptional information was carried with rice MPSS datebase in seeding stage and tilling stage.Results: In this experiment, TLP-specific antibodies were generated, and the expression patterns of TLPs in rice leaves at different developmental stages were revealed by Western Blotting (WB). The expression of TLPs at different time points in the incompatible responses, mediated by bacterial blight resistant gene Xa21 against Xanthomonas oryzae pv. oryzae (Xoo), were also surveyed. Variations were observed for the expression of TLP2, TLP7, TLP8 and TLP9 in the incompatible responses. And their expression patterns also varied among compatible interaction, incompatible interaction and mock control. The results indicated that the expression of TLP proteins differ from each other. Taken together, these results provided important clues for the interpretation of TLP function in rice, especially in the interaction between rice and Xoo.Conclusions: By the expression profiling of 14 Tubby-like proteins in different stages of rice leaves, we found that the proteins have three expression patterns. This experiment results revealed the in rice leaves at different growth stages and the correlation between the proteins and the growing process in a direct and relative quantitative way, and the diversification of protein expression of Tubby-like proteins provided significant information for further research of their function. It also provided important scientific basis for chloroplast function in vital processes such as rice normal growth and response of adversity.