Protective Effects Of Lycium Barbarum Polysaccharides And Quercetin On Testicle Injuries Induced By Bisphenol A In Mice

The study was carried out to evaluate the effects of lycium barbarum polysaccharides (LBP) and quecretin (Que) on testicle injuries induced by bisphenol A (BPA) in mice testis. Testis and epididymis were weighted, organ coefficients were calculated. The pathological changes of the testicle tissue were observed under light microscope. The enzyme activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and the malonaldehyde (MDA) contents in serum and the contents of testosterone (T), gonadotropin-releasing hormone (GnRH) and luteinizing hormone (LH) in serum were detected by ELISA. The aim of the study was to look for the protective measures in male reproductive system induced by environmental estrogens.The experiment was divided into three parts. The purpose of part one was to investigate the harmful effects of bisphenol A on adult male reproductive system. The objective of part two was to evaluate the protective effects of LBP on BPA induced testicle injury in mice. The third part was to explore the protective effects of quercetin on spermatogenic disturbance in mice induced by BPA, and to explore the best effective dose of Que.â… To study the harmful effects of BPA on adult male reproductive systemTo study the effects of BPA on adult male reproductive system, 40 puberty mice were equally divided into 4 groups, including a control group and 3 BPA treated groups (10,20, 50 and 100 mg/kg, respectively). BPA was intraperitoneally injected once a day for 7 days, while mouse in control group received equal olive oil. Testis and epididymis were weighted, organ coefficients were calculated and histopathological changes were examined. The contents of T and LH in serum were detected by ELISA. Results showed that there were significant differences in control group compared with BPA groups in the organ weights and the organ coefficients of testis and epididymis (P<0.05, P<0.01). While germ cells in BPA treated groups were detached from basement membrance of seminiferous tubules and Sertoli cells. The spermatogenic cells array loosely and disorderly. The contents of T and LH were significantly reduced in BPA treated mouse compared with the negative control group (P<0.05), the levels of T and LH in 50, 100 mg/kg groups were remarkably decreased than those of the control group (P<0.01). Conclusion: BPA could damage the reproductive system on adult male mice. â…¡To evaluate the protective effects of LBP on BPA induced testicle injury in miceTo evaluate the effects of LBP on BPA induced testicle injury in mice. Methods: Adult male mouse were divided into five groups, the group A was control group and given olive oil, the mouse in other 4 groups were injected with 20 mg/kg of BPA daily for 7 days. At the same time, the mouse in C, D, E groups were administrated with 50, 100, 200 mg/kg of LBP, the mouse in group A and B were given equivalent amount of normal saline daily for 7 days. After the treatment, testis and epididymis were weighted, organ coefficients were calculated and histopathological changes were examined. SOD and GSH-Px activities, MDA contents were detected. The contents of T, LH and GnRH in serum were detected by ELISA. Results: After supplement with 200 mg/kg LBP, the weights of testis and epididymis, the organ coefficients of testis and epididymis significantly increased compared with BPA group. The seminiferous tubules in LBP treated mouse were lined up in order. The antioxidant enzyme activities of SOD and GSH-Px in blood serum were significantly elevated in LBP treated groups. MDA contents were significantly decreased in LBP groups. There were significant increases in the levels of T, LH and GnRH in LBP groups compared with the BPA group. The levels of T in group D (100 mg/kg LBP) were significantly higher than that in the BPA group. The levels of LH in 100, 200 mg/kg LBP groups were higher than in the BPA group. The levels of GnRH in 50, 100 mg/kg LBP groups were higher than in the BPA group. Conclusion: The results indicate that LBP protects the mice from BPA induced reproductive damage. LBP had visible effects in attenuating the reproductive damage induced by BPA.â…¢To study the effects of Que on spermatogenic disturbance in mice induced by BPATo study the effects of Que on spermatogenic disturbance in mice induced by BPA, and to explore the best effective dose of Que. Methods: Fifty male mouse were randomly divided into five groups. Group A (n = 10) were normally raised, mouse in Group B, C, D and E were injected with 50 mg/kg of BPA daily for 7 days. Meanwhile, group C, D and E received Que through intragastric administration in different concentrations (100, 200 and 400 mg/kg.d respectively), and the rest two groups received physiological saline. Then the physical sign and body weight of mouse were recorded, the weight of testes, epididymis, seminal vesicle were measured. SOD and GSH-Px activities, MDA contents were detected. The levels of T, LH and GnRH were determined by enzyme-linked immunosorbent assay. Histological observation of the testis tissues were done under the microscope. Results: Compared with those of Group B, the weight of body, testis and epididymis seminal vesicle in Group D (200 mg/kg) were improved obviously (P<0.05 or P<0.01) and close to those in Group A. The body weight in Group C and E were improved, but could not restore to the normal level. The levels of the serum hormone in Group D recovered to the levels showing in normal animal (P>0.05) and better than those in Group C and E. The layers of spermatogenic cells, the sperm number of spermatogenic tubules in Group C, D and E were superior to those of Group B, especially in Group D (200 mg/kg). Conclusion: Que could promote the recovery of spermatogenic disturbance in mice induced by BPA.Present study indicated that BPA could induce the testicular injury on the adult male mice. However, both LBP and Que could exert their protective actions on adult male reproductive system against BPA-induced testicular damage. The protective effects of LBP and Que may be related to the increasing activities of antioxidant enzyme system and restoring the reproductive hormones to the normal levels.