| Pepsinogen (PEP) and liportein lipase (LPL) are critical enzymes in the dietary protein and lipid levels, digestibility and metabolism. In the present study, analyses of single nucleotide polymorphisms (SNPs) on PEP and LPL in Siniperca chuatsi based on population sequencing by direct sequencing (DS) and created restriction site PCR (CRS-PCR), was firstly carried out. in order to investigate the relationship of the two genes polymorphisms and phenotype with special feed habit domestication. using the methods of CRS-PCR and RFLP, polymorphisms in PEP ranging from exon 5 to exon 8 (introns included) were analyzed, and two SNP sites in exon 7 (C1T, C52T) were identified, both of which were synonymous mutations.the analysis between domesticated and undomesticated populations by Chi-square test among two SNP sites show not significantly different (P>0.05). Five diplotypes were constructed based on two SNP sites in the experiment population. Chi-square test indicated that there was significant association between diplotypel and diplotype5 in domesticated and undomesticated populations (P<0.05).Using the methods of DS, polymorphisms in LPL ranging from exon 5 to exon 8 (introns included) were analyzed, and three SNP sites in exon 7 (A25T, G26T, C29G) were identified, two of which are non-synonymous mutations. the analysis between domesticated and undomesticated populations by Chi-square test among three SNP sites show not significantly different (P>0.05). Five diplotypes were constructed based on three SNP sites in the experiment population. Chi-square test indicated that there was significant association between diplotype2 in domesticated and undomesticated populations (P<0.05).These genes can consider as genetic markers for feed habit domestication phenotype in Siniperca chuatsi. In addition, this study laid the foundation for developement of feeding domesticated cultivation of Siniperca chuatsi research in future.
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