Isolation And Characterization Of Polymorphic SSRs Markers In Three Siniperca Chuatsi Population

To protect the germplasm resources of nature Siniperca chuatsi and acceleratethe process of genetic breeding,600unigenes were got from the Siniperca chuatsiunigenes library sequenced by High-throughput transcriptome, from which120SSRs locus were searched.91pairs of SSRs primers amplified clear PCR productsteadily. Each individual of three populations sampled from Changde, Huaihua andChibi was extracted into DNA, which was used for PCR amplification with thesynthetic SSR primers. Finally,22polymorphic SSR primers were screened byagarose gel electrophoresis and polyacrylamide gel electrophoresis. Then geneticpolymorphic analysis was carried out in three population with these22polymorphicSSRs primers. The results indicated that these twenty-two makers showed abundantpolymorphism,134alleles were found in all the SSR markers, the allele number perlocus ranged from4to8, the mean observed and expected heterozygosities were0.6754and0.7487, respectively. And the mean polymorphism information contentwas0.7010. In addition, the genetic index in three populations suggestes betterpolymorphism in each population. Afer F-test, the mean Fst value was0.0383, whichindicates low differentiation was existed in these three populations. The geneticidentity of Changde and Huaihua was the highest and the genetic distance was thelowest, while those of Changde and Chibi were reversed, respectively, which isconsistent with their living regions. As the Changde and Huaihua populations werefrom Yuanjiang River located in Hu’nan province, while Chibi population was fromLushui reservoir in Hubei province.