| Kandelia candel is one of salt-tolerant mangrove species and habitats in seaside area. Preliminary studies have shown that KcBURP and KcC2H2 zinc finger gene of Kandelia candel seedlings were significantly up-regulated under salt stress, suggesting that they contributed to salt tolerance of Kandelia candel.In the presented study, the cDNA coding for the open reading frames (ORF) of KcBURP and KcC2H2 zinc finger genes of Kandelia candel were obtained by a PCR approach using the primers designed from the sequence information of Microarry cDNA. Sequencing analysis indicate that the ORF of KcBURP has a size of 1131bp, encoding for a protein of 375 amino acid with an isoelectric point of 9.07 and a molecular weight of 39886 Da.The ORF of KcC2H2 zinc finger gene has a size of 738bp, encoding for a protein of 245 amino acid with an isoelectric point of 8.65 and a molecular weight of 26174Da.The gene construct of the KcBURP under the control of constitutive promoter pCAMBIA-2300 was transformed into tobacco. Ten individual KcBRP-transformed tobacco plants were identified by PCR. The PCR result show that eight of ten plants were KcBURP-positive. While there were only two plants showed over expression when the KcBURP-transformed tobacco plants were tested by RT-PCR. Salt tolerance examinations show that the KcBURP-transformed tobacco plants grew better than the wildtype under 150 mM NaCl. Furthermore, net photosynthetic rate of KcB URP-transformed tobacco plants were higher than the wildtype under 100 and 200 mM of NaCl saline, although the leaf photosynthesis was reduced by salt treatment. It is noting that the photosynthetic rate of KcBURP-transformed tobacco plants recovered rapidly than the'wildtype after the salt relief. Our result suggest that KcBURP gene is beneficial for plant salt tolerance. The gene construct of KcC2H2 the under the control of constitutive promoter pK7WG2D was transformed into tobacco. We identified seven KcC2H2-transformed tobacco plants by means of PCR test.
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