Differential Protein Analysis Of Salt Stress Response In Kandelia Candel Roots

Mangrove plants are grown in tropical and subtropical coastal intertidal plant communities.They are important to maintain the ecological balance of coastal vegetation types. Mangrove is a halophyte or salt-tolerant plants, not only grown in the high salinity of the soil matrix, but also soak by periodic high-salt water. In the long process of evolution, mangrove species form a set of different salt-tolerant mechanism from terrestrial plants and aquatic plants.On the basis of the previous researsh about salt tolerance mechanism on mangroves in the morphological, physiological and biochemical, we continue the study in the physiological and molecular level, in particular the post-genome era of proteomics on the salt tolerance of Kandelia candel mangrove. Based on physiological and biochemical indicators of the roots under salt stress of K. candel,two dimensional electrophoresis, mass spectrometry, bioinformatics comparative were conducted to analysis the differentially expressed proteins of K. candel mangrove plants'root under salt stress .After the classification of the differentially expressed proteins,we study the relationship between salt-tolerant mechanism,and then reveal salt-tolerant protein function network of mangrove.Mature K.candel mangrove grow to 4-6 leaves in the Hoagland nutrient solution as mother liquor paired with 4 different NaCl concentration (0mmol / L (control), 150 mmol / L, 300 mmol / L, 600 mmol / L) for 3 days (ie, 72h), then collected the roots of K. candel seedlings for the determination of physiological and biochemical and protein extraction. In the study,we mainly determined proline content, MDA content, SOD content of the root under different salt stress. Two-dimensional electrophoresis (2-DE) was conducted to separate the protein, four two-dimensional electrophoresis profiles obtained after scanning two-dimensional electrophoresis map,PDQuest software, protein spot detection and statistical analysis,and then identify respectively differentially expressed proteins of K. candel mangrove plants'root under salt stress. 25 spots with quantitative changes more than two times were identified by MALDI- TOF/TOF-MS). 7 proteins showed up-regulation ,2 showed down-regulation, and 16 changed irregularly.The identified rate was 76%,and 19 proteins were successfully identified. Their functions are involved in energy metabolism (31%), antioxidant (11%), stress-related protein (11%),protein metabolism (11%), nitrogen metabolism-related protein (5%),nucleic acid metabolism (5%), unclassification of protein (26%).The study was on the basis of the previous researsh about salt tolerance mechanism on K. candel in the morphological, physiological and biochemical,and we make use of proteomics methods and technologies to compare differentially expressed proteins of roots that were exposed in different NaCl concentration. Through the process of functional analysis of differentially expressed proteins, in order to provides the basis of molecular biology for cultivating salt-tolerant plant species, that will be of great significance.to solve the problem of soil salinization.