| Top buds of Populus. ussuriensis Kom. was used as experimental material, and total RNA was extracted from the top buds, we used Creator. SMART. cDNA Library Construction Kit to construction the cDNA Library. We Sequenced independent clones, used the methods of Expressed Sequence Tags (ESTs) for gene expression analysis.The library qualification evaluation showed, The library had 7.2×106 independent clones, fragments with an average of length about more than 0.5 kb in length, recombination rate of 97%. cDNA library from top buds of Populus ussuriensis Kom. was constructed successfully.Sequenced 3045 independent clones,2218 high quality expressed sequence tags (ESTs) were discover from 1944 unigene. There were 180 contig and 1764 singletons in the 1944 unigene.1944 unigene were annotated by blastX,496 in which were annotated with definite function, amounting to 25.41% in all unigene.822 were considered as novel gene for little homology, amounting to 42.28% in all unigene.624 in which were annotated with unknown protein, amounting to 32.10% in all unigene. There were some genes for growth in Full-length cDNA library from top buds of Populus ussuriensis Kom. the associated DNA genetic resources in this study will be a useful resource for the further study in understanding the molecular mechanisms of growth.
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