Genetic Variation Analysis Of Corn Progenies With Starch Branching Enzyme Gene By The Antisense And Interference

Genetically modified maize materials were tested and genetic variation of GM maize Progenies was analyzed. It provides the basis for selection of variation materials in transgenic progeny.The study shows that the general agronomic traits of GM maize for T2 and T3 generation are better than non-transgenic plants and traits vary greatly. Transgenic mature plants of T2 generation were all gained. For the T3 generation, we should select traits focusing on the larger coefficient of variation.In agronomic traits of different transgenic plants, the treatment variation of stem diameter, ear height, grain weight and ear weight reaches a highly significant level, kernel weight variation is significant, and the remaining traits have no significant differences.In mature grain of transgenic maize for T2 generation, coefficient of variation of starch and protein content are larger than the control, coefficient of variation of fat content is lower than the control. The average value of fat, starch and protein content is higher than the control, fat content is in a highly significant level and protein content is in significant level. It indicates that the transgenic quality traits are very different and their progeny have a choice potential for high protein and high fat..For the transgenic lines of T3 generation, average value of starch, protein and fat has no significant difference with the control. For the fat of 28-B lines, the starch of 53-B lines and 29-B lines, the protein of 29-B lines, their coefficient of variation is larger than control, so it is more effective to select the high-fat plant derived from 28-B plant, to select high-starch plant derived from 29-B and 53-B, to select high protein plant in the 29-B-derived lines.Grain amylose content of 29-B,31-B,31-A,28-B are significant or highly significant with the control. It is more effective to select high amylose content plant from lines of the above plant. The main agronomic traits of transgenic plants have complex correlation, variation of a trait causes variation of other traits at the same time. For T2 generation, ear length has a correlation with stem diameter, ear diameter, kernel number per row, bald length, cob diameter, cob rate, protein. Cob weight has no significant differences from other traits. For T3 generation, ear height has correlation with plant height, but no correlation with other traits. Ear length is not relational with plant height, ear height, bald length, cob rate and has the correlation with other traits.The test for goodness-of-fit was carried out. The negative and positive number of plant is in the ratio of 15:1 for T2 transgenic plants by the test for goodness-of-fit. Except for 53-B lines, the negative number and positive number of other lines are not in the ratio 3:1 for T3 transgenic plants by the test for goodness-of-fit. Except for 31-A and 53-B lines, the negative number and positive number of other strains is in the ratio of 15:1. They do not meet the Mendelian law.