Construction Of Fingerprinting And Analysis Of Genetic Diversity With SSR Marker For Tobacco Core Germplasm

Tobacco is an important economic crop in China, tobacco core germplasm have been contributing a lot not only to tobacco production but also to varietal improvement as germplasm resource. Fingerprinting map construction is important to use tobacco germplasm resource rightly, identify variety and protect intellectual property rights. In this study, SSR markers were employed to analysis 441 tobacco core germplasm. Some primers with high polymorphism were selected out, DNA fingerprints database of tobacco core germplasm was constructed and the genetic diversity was analyzed. The main results:1. Based on 10 N.tabacum germplasm distinct in genetic background, 8 pairs of primers which were PT20189,PT20287,PT20372,PT30380,PT20202,PT30403,PT20213,PT20242 were selected from 286 pairs to analyze 381 N.tabacum germplasm, the 8 primer pairs amplified a total of 85 polymorphic alleles among the 381 N.tabacum germplasm, polymorphic alleles amplified by each pair of primer ranged from 7 to 22, with a mean of 10.6. The polymorphic information content values (PIC) ranged from 0.63 to 0.88, with a mean of 0.81. UPGMAcluster analysis showed that the 8 pairs of primers could distinct all the 381 N.tabacum germplasm, every tobacco germplasm has a unique fingerprinting map.2. Based on 4 wild tabacco germplasm distinct in genetic background, 4 pairs of primers which were TPS-111,TPS-123,1TPS-124,TPS-126 were selected from 100 pairs to analyze 30 wild tobacco germplasm, the 4 primer pairs amplified a total of 25 polymorphic alleles among the 30 wild tobacco core germplasm, polymorphic alleles amplified by each pair of primer ranged from 5 to 8, with a mean of 6.2. The polymorphic information content values ranged from 0.60 to 0.81, with a mean of 0.70. UPGMAcluster analysis showed that the 4 pairs of primers could distinct all the 30 wild tobacco germplasm, and so every wild tobacco germplasm has a unique fingerprinting map.3. The data of the study showed that genetic diversity of tobacco core germplasm is high in China .UPGMA cluster analysis showed that genetic diversity of different types germplasm is obvious among N.tabacum, genetic diversity of wild tobacco core germplasm is unrelated with country of origin.