| The Effect of Bovine-source Prebiotics GroBiotic?-A on Growth Performance, Feed Efficiency and Digestibility of Nutrients in Diets of Siberian Sturgeon (Acipenser baerii Brandt)An 8-week experiment was conducted to study the Effect of Bovine-source Prebiotics GroBiotic?-A on growth performance, feed efficiency and digestibility of nutrients in diets of Siberian Sturgeon (Acipenser baerii Brandt). Siberian sturgeons (initial body weight =75±0.1g) were randomly divided into 16 tanks for 4 treatment with 18 fish/tank in a recirculation system. The basal diet was formulated with 36% crude protein, 11% crude lipid, in which four levels of GroBiotic?-A 0% (G0), 0.4% (G0.4), 0.8% (G0.8), 2% (G2) were included. Y2O3 (0.1%) was added in each diet as inert marker for digestibility determination. Weight gain rate (WGR) was not different, but feed conversion ratio (FCR) was reduced with higher GroBiotic?-A inclusion levels, and FCR of G2 group showed lowest FCR. Apparent digestibility coefficients (ADC) of dry matter and protein were not significantly different, but fish fed G2 diet showed significantly higher ADC of Lys, His, Leu, Thr and Val (P<0.05). 2% of GroBiotic?-A in Siberian sturgeon diet will increase feed efficiency and essential amino acids digestibility.The Effect of GroBiotic?-A upon the Growth Performance, nonspecific immunity of Siberian Sturgeon and challenged in situ with Aeromonas hydrophilaThe objective of the study was to evaluate the effects of Bovine-source Prebiotics GroBiotic?-A upon the growth performance, non-specific immunity of Siberian Sturgeon (Acipenser baerii Brandt) before and after a challenge in situ with Aeromonas hydrophila.During 8 weeks growth trial,288 Siberian sturgeons (initial body weight =75±0.1g) were randomly divided into 16 tanks for 4 treatment with 18 fish/tank in a recirculation system. The basal diet was formulated with 36% crude protein, 11% crude lipid, in which four levels of GroBiotic?-A 0% (G0), 0.4% (G0.4), 0.8% (G0.8), 2% (G2) were included. (Results) The results show that G2 have the significantly higher WGR than G0 (P<0.05); Feed intake (FI) of G2 was significantly lower than that of G0; Fish of G0.4 and G0.8 groupsshowed lower FCR than G0.4, and no significantly difference with other groups (P<0.05); Before challenged in situ with A. hydrophila. G0.8 have significant higher of plasma lysozyme level than other groups; G2 have highest of the plasma complement 3 in all four groups; G0.8 and G2 have significant higher of MPO than G0. After challenged in situ with A. hydrophila. G0.4 have lower of superoxide anion level than G0; G0.8 have significant higher of plasma lysozyme level than other groups; G2 have the highest MPO level than other groups. In conclusion, 2% of GroBiotic?-A could improve the Siberian sturgeon's growth performance and immunity characteristics and improve resistance of A. hydrophila. Compared effect of GroBiotic?-A upon the Growth Performance, Nonspecific Immunity of Siberian Sturgeon and Challenged in situ with Aeromonas hydrophila under Normal or Chronic Harassing Stress conditionsAn 8-week experiment was conducted to study the paired effect Bovine-source prebiotics GroBiotic?-A on growth performance, feed efficiency and digestibility of dietary nutrients and immune response before and after chanllenge by Aeromonas hydrophila on Siberian Sturgeon (Acipenser baerii Brandt).Under normal or chronic harassing stress conditions. Siberian sturgeons (initial body weight =75±0.1g) were randomly divided into 16 tanks for 4 treatment with 18 fish/tank in a recirculation system. The basal diet was formulated with 36% crude protein, 11% crude lipid, which divided into 2×2 treatments which stress and 2% GroBiotic?-A, which are C(control of normal), G (normal treatment with GroBiotic?-A), S (control of stress) and G×S (stress treatment with GroBiotic?-A). The chronic harassing stress could significantly lower the non-specific immunity (plasma lysozyme, complement 3 and superoxide anion) of Siberian Sturgeon. There are no significant variance among groups on final body weight and FCR, but could significant improved WGR and FI (P>0.05). G group have significantly higher WGR than C group and FI and FCR significantly lower than S group. When we added 2% GroBiotic?-A into Siberian Sturgeon's feed, it could significantly improve the plasma lysozyme and MPO level than groups without it, the same result can repeat when the sturgeon challenged with Aeromonas hydrophila on plasma lysozyme, superoxide anion and MPO. Under the stress and GroBiotic?-A, complement 3 of G group significantly higher than other groups , plasma lysozyme, MPO and NBT of G×S group signifiantly than other groups before challenged with Aeromonas hydrophila. After challenged with Aeromonas hydrophila, superoxide anion of C group significantly higher than G×S group, complement 3 of G×S group significantly higher than S group; MPO group significantly higher than C group; NBT of G×S group significantly higher than others group. According to the results, we got that the GroBiotic?-A could improve the Siberian sturgeon's growth performance, immunity characteristics and improve resistance of Aeromonas hydrophila under the stress. |
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